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The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella
April 2010
April 2010
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Abstract
The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune
response and in the regulation of DNA damage-induced apoptosis. In the present study,
the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenopharyngodon
idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF)
of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9%
identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and
95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association
domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075bp consisting
of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined.
The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV)
induced the expression of gcIRF-5 in spleen and head kidney.