Using buoyant mass to measure the growth of single cells

Godin, Michel; Delgado, Francisco Feijó; Son, Sungmin; Grover, William H.; Bryan, Andrea K; Tzur, Amit; Jorgensen, Paul; Payer, Kris; Grossman, Alan D.; Kirschner, Marc W; Manalis, Scott R.

doi:10.1038/nmeth.1452

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Using buoyant mass to measure the growth of single cells

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Author(s): Michel Godin ¹ , Francisco Feijó Delgado ¹ , Sungmin Son ³ , William H. Grover ¹ , Andrea K. Bryan ¹ , Amit Tzur ⁴ , Paul Jorgensen ⁴ , Kris Payer ⁶ , Alan D. Grossman ⁷ , Marc W. Kirschner ⁴ , Scott R. Manalis ¹ ^, ³

Publication date (Electronic): 11 April 2010

Journal: Nature methods

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Abstract

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.

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Author and article information

Journal

Journal ID (nlm-journal-id): 101215604

Journal ID (pubmed-jr-id): 32338

Journal ID (nlm-ta): Nat Methods

Title: Nature methods

ISSN (Print): 1548-7091

ISSN (Electronic): 1548-7105

Publication date Nihms-submitted: 31 March 2010

Publication date (Electronic): 11 April 2010

Publication date (Print): May 2010

Publication date PMC-release: 1 November 2010

Volume: 7

Issue: 5

Pages: 387-390

Affiliations

[1 ] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

[3 ] Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

[4 ] Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA

[6 ] Microsystems Technology Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

[7 ] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Author notes

Correspondence should be addressed to S.R.M.( scottm@ 123456media.mit.edu )

[*]

co-1st authors

[2]

Present address: Department of Physics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada

[5]

Present address: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON M5S 3E1, Canada

Article

Manuscript ID: nihpa190936

DOI: 10.1038/nmeth.1452

PMC ID: 2862099

PubMed ID: 20383132

SO-VID: ab2a20e0-644b-4699-9bb5-dc637e244b35

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