Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Initiation complex formation on Euglena chloroplast 30S subunits in the presence of natural mRNAs.

Nucleic Acids Research

Base Sequence, Chloroplasts, metabolism, Escherichia coli, genetics, Euglena, Genetic Vectors, Genomic Library, Kinetics, Molecular Sequence Data, Nucleic Acid Hybridization, ultrastructure, Oligonucleotide Probes, Peptide Chain Initiation, Translational, Protein Biosynthesis, RNA, Messenger, RNA, Transfer, Amino Acyl, RNA, Transfer, Met, Ribosomes, Animals

Read this article at

ScienceOpenPMC
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      An in vitro system has been developed that allows the formation of translation initiation complexes with Euglena chloroplast 30S ribosomal subunits and natural mRNAs. For these experiments two regions of the Euglena chloroplast genome have been cloned behind the T7 transcriptional promoter and the corresponding RNAs synthesized in vitro. These mRNAs are capable of forming initiation complexes with chloroplast 30S subunits in the presence of fMet-tRNA and E. coli initiation factors. Deletion of the normal translation start site results in a message that is no longer recognized by the chloroplast subunits suggesting that the correct AUG initiation codon on the mRNA is being selected by the small ribosomal subunit. Initiation complex formation with the chloroplast 30S subunits is specific for chloroplast mRNAs and mRNA from the phage MS2 is not active in this system.

      Related collections

      Author and article information

      Journal
      2690007
      335210

      Comments

      Comment on this article