Altered electrochemical properties of iron oxide nanoparticles by carbon enhance molecular biocompatibility through discrepant atomic interaction

Abstract

Recent advancement in nanotechnology seeks exploration of new techniques for improvement in the molecular, chemical, and biological properties of nanoparticles. In this study, carbon modification of octahedral-shaped magnetic nanoparticles (MNPs) was done using two-step chemical processes with sucrose as a carbon source for improvement in their electrochemical application and higher molecular biocompatibility. X-ray diffraction analysis and electron microscopy confirmed the alteration in single-phase octahedral morphology and carbon attachment in Fe ₃O ₄ structure. The magnetization saturation and BET surface area for Fe ₃O ₄, Fe ₃O ₄/C, and α-Fe ₂O ₃/C were measured as 90, 86, and 27 emu/g and 16, 56, and 89 m ²/g with an average pore size less than 7 nm. Cyclic voltammogram and galvanostatic charge/discharge studies showed the highest specific capacitance of carbon-modified Fe ₃O ₄ and α-Fe ₂O ₃ as 213 F/g and 192 F/g. The in vivo biological effect of altered physicochemical properties of Fe ₃O ₄ and α-Fe ₂O ₃ was assessed at the cellular and molecular level with embryonic zebrafish. Mechanistic in vivo toxicity analysis showed a reduction in oxidative stress in carbon-modified α-Fe ₂O ₃ exposed zebrafish embryos compared to Fe ₃O ₄ due to despaired influential atomic interaction with sod1 protein along with significant less morphological abnormalities and apoptosis. The study provided insight into improving the characteristic of MNPs for electrochemical application and higher biological biocompatibility.

Graphical abstract

Highlights

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  Carbon modification of octahedral shaped magnetic nanoparticles (MNPs) was done using sucrose.

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  The magnetization saturation and BET for Fe ₃O ₄, Fe ₃O ₄/C and α-Fe ₂O ₃/C was 90, 86, 27 emu/g and 16, 56, 89 m ²/g.

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  Carbon modified α-Fe ₂O ₃ induce less ROS in exposed zebrafish embryos.

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  Carbon modified Fe ₃O ₄ despair influential atomic interaction with zebrafish Sod1 protein.

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  Carbon modified Fe ₃O ₄ exhibit less morphological abnormalities and apoptosis.