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      Phaser crystallographic software

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          Abstract

          A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.

          Abstract

          Phaser is a program for phasing macromolecular crystal structures by both molecular replacement and experimental phasing methods. The novel phasing algorithms implemented in Phaser have been developed using maximum likelihood and multivariate statistics. For molecular replacement, the new algorithms have proved to be significantly better than traditional methods in discriminating correct solutions from noise, and for single-wavelength anomalous dispersion experimental phasing, the new algorithms, which account for correlations between F + and F , give better phases (lower mean phase error with respect to the phases given by the refined structure) than those that use mean F and anomalous differences Δ F. One of the design concepts of Phaser was that it be capable of a high degree of automation. To this end, Phaser (written in C++) can be called directly from Python, although it can also be called using traditional CCP4 keyword-style input. Phaser is a platform for future development of improved phasing methods and their release, including source code, to the crystallographic community.

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          Author and article information

          Journal
          J Appl Crystallogr
          J. Appl. Cryst.
          Journal of Applied Crystallography
          International Union of Crystallography
          0021-8898
          01 August 2007
          13 July 2007
          13 July 2007
          : 40
          : Pt 4 ( publisher-idID: j070400 )
          : 658-674
          Affiliations
          [a ]Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK
          [b ]Lawrence Berkeley National Laboratory, One Cyclotron Road, Bldg 64R0121, Berkeley, CA 94720-8118, USA
          [c ]Daresbury Laboratory, Warrington WA4 4AD, UK
          Author notes
          Correspondence e-mail: ajm201@ 123456cam.ac.uk
          [‡]

          Present address: Department of Applied Mathematics and Theoretical Physics, University of Cambridge, UK.

          Article
          he5368 JACGAR S0021889807021206
          10.1107/S0021889807021206
          2483472
          19461840
          © International Union of Crystallography 2007

          This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.

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          Research Papers

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