The Role of ( E)-2-octenyl Acetate as a Pheromone of Bagrada hilaris (Burmeister): Laboratory and Field Evaluation

Abstract

The pentatomid bug Bagrada hilaris is a key pest of brassicaceous crops in several areas of the world. Previous studies suggest that mate location of this species is mediated by volatile chemicals produced by males, among which the main compound is ( E)-2-octenyl acetate. However, the possible attraction of males, females, and nymphs to this compound has not yet been specifically tested. In this study, we tested the response of B. hilaris females, males, and nymphs to ( E)-2-octenyl acetate using an electroantennogram (EAG) and olfactometer in the presence or absence of a host plant. Moreover, ( E)-2-octenyl acetate as an attractant lure in field trap bioassays was evaluated. EAG recordings showed that this compound evokes antennal responses in B. hilaris females. Olfactometer behavioral responses showed that females and nymphs were attracted to ( E)-2-octenyl acetate, while males showed no attraction. In the field trap bioassays, captures were obtained in traps baited with 5 and 10 mg of ( E)-2-octenyl acetate, while in traps loaded with 2 mg and control traps, there were no recorded catches. These results suggest the involvement of ( E)-2-octenyl acetate in intraspecific interactions of this species.