+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Interleukin-1β and Interleukin-6 Specifically Increase the Release of Prostaglandin E 2 from Rat Hypothalamic Explants in vitro

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          It has previously been shown that the cytokines interleukin-1β and interleukin-6 (IL-1β and IL-6) stimulate directly the release of corticotrophin-releasing-hormone-41 from the rat hypothalamus in vitro, while IL-1β can also stimulate the release of somatostatin. These effects can be antagonized by drugs which block prostaglandin (PG) synthesis. PGs are also involved in the control of hypothalamic neuropeptides by other neuron transmitters. In the present study, we have characterized the production of PGs from the rat hypothalamus in vitro, and investigated the effects of IL-1β and IL-6, as well as the neurotransmitters norepinephrine, acetylcholine and 5-hydroxytryptamine, on the acute release of PGs, using a well-validated acute hypothalamic incubation system. The rate of release of PGs [PGE<sub>2</sub>, PGF<sub>2α</sub> 6-keto-PGF<sub>1α </sub>(6KPGF<sub>1α</sub>) and thromboxane B<sub>2</sub> (TXB<sub>2</sub>) in the medium was found to stabilize after 60 min of preincubation and thereafter remain constant, with TXB<sub>2</sub> being the predominant species. Twenty-minute incubation in the presence of human recombinant IL-1β or IL-6, in the dose range 1-100 U/ml, had no effect on the release of PGF<sub>2α</sub>, 6KPGF<sub>1α</sub> or TXB<sub>2</sub>; however, the release of PGE2 was significantly increased by both IL-1β and IL-6. The effect of IL-1β was antagonized by both indomethacin and dexamethasone. None of the other neurotransmitters tested had any effect on the release of any of the PGs. It is concluded that the regulation of hypothalamic PG release may be investigated by means of acute rat hypothalamic explants, as has previously been shown for hypothalamic peptides and amines. Furthermore, IL-1β and IL-6 specifically stimulate PGE2 release, possibly implicating this PG in certain of the hypothalamic effects of IL-1β and IL-6.

          Related collections

          Author and article information

          S. Karger AG
          07 April 2008
          : 56
          : 1
          : 61-68
          aDepartment of Pharmacology, Catholic University Medical School, Rome, Italy; bDepartment of Endocrinology, St. Bartholomew’s Hospital, London, UK
          126209 Neuroendocrinology 1992;56:61–68
          © 1992 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Original Paper


          Comment on this article