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      Propeller-type parallel-stranded G-quadruplexes in the human c-myc promoter.

      Journal of the American Chemical Society

      DNA, chemistry, Genes, myc, genetics, Guanine, Humans, Models, Genetic, Nucleic Acid Conformation, Promoter Regions, Genetic, Transcription, Genetic

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          Abstract

          The nuclease-hypersensitivity element III1 in the c-myc promoter is a good anticancer target since it largely controls transcriptional activation of the important c-myc oncogene. Recently, the guanine-rich strand of this element has been shown to form an equilibrium between G-quadruplex structures built from two different sets of G-stretches; two models of intramolecular fold-back antiparallel-stranded G-quadruplexes, called "basket" and "chair" forms, were proposed. Here, we show by NMR that two sequences containing these two sets of G-stretches form intramolecular propeller-type parallel-stranded G-quadruplexes in K(+)-containing solution. The two structures involve a core of three stacked G-tetrads formed by four parallel G-stretches with all anti guanines and three double-chain-reversal loops bridging three G-tetrad layers. The central loop contains two or six residues, while the two other loops contain only one residue.

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          Journal
          15250723
          10.1021/ja048805k

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