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      Benthic algae assessments in the EU and the US: Striving for consistency in the face of great ecological diversity

      , , , , , ,
      Ecological Indicators
      Elsevier BV

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          Performance comparison of benchtop high-throughput sequencing platforms.

          Three benchtop high-throughput sequencing instruments are now available. The 454 GS Junior (Roche), MiSeq (Illumina) and Ion Torrent PGM (Life Technologies) are laser-printer sized and offer modest set-up and running costs. Each instrument can generate data required for a draft bacterial genome sequence in days, making them attractive for identifying and characterizing pathogens in the clinical setting. We compared the performance of these instruments by sequencing an isolate of Escherichia coli O104:H4, which caused an outbreak of food poisoning in Germany in 2011. The MiSeq had the highest throughput per run (1.6 Gb/run, 60 Mb/h) and lowest error rates. The 454 GS Junior generated the longest reads (up to 600 bases) and most contiguous assemblies but had the lowest throughput (70 Mb/run, 9 Mb/h). Run in 100-bp mode, the Ion Torrent PGM had the highest throughput (80–100 Mb/h). Unlike the MiSeq, the Ion Torrent PGM and 454 GS Junior both produced homopolymer-associated indel errors (1.5 and 0.38 errors per 100 bases, respectively).
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            A coded checklist and ecological indicator values of freshwater diatoms from The Netherlands

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              Biological Integrity: A Long-Neglected Aspect of Water Resource Management

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                Author and article information

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                Journal
                Ecological Indicators
                Ecological Indicators
                Elsevier BV
                1470160X
                February 2021
                February 2021
                : 121
                : 107082
                Article
                10.1016/j.ecolind.2020.107082
                bd54e6e3-3b04-4c7f-a5c5-85850e649ccf
                © 2021

                https://www.elsevier.com/tdm/userlicense/1.0/

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