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      Energy landscape roughness of the streptavidin-biotin interaction.

      Journal of molecular recognition : JMR

      metabolism, chemistry, Streptavidin, Protein Binding, Models, Theoretical, Models, Molecular, Microscopy, Atomic Force, Kinetics, Energy Metabolism, Biotin

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          Abstract

          Molecular interactions between receptors and ligands can be characterized by their free energy landscape. In its simplest representation, the energy landscape is described by a barrier of certain height and width that determines the dissociation rate of the complex, as well as its dynamic strength. Some interactions, however, require a more complex landscape with additional barriers and roughness along the reaction coordinate. This roughness slows down the dissociation kinetics of the interaction and contributes to its dynamic strength. The streptavidin-biotin complex has been extensively studied due to its remarkably low dissociation kinetics. However, single molecule measurements from independent experiments showed scattered and disparate results. In this work, the energy landscape roughness of the streptavidin-biotin interaction was estimated to be in the range of 5-8kBT using dynamic force spectroscopy (DFS) measurements at three different temperatures. These results can be used to explain both its slow dissociation kinetics and the discrepancies in the reported force measurements. Copyright (c) 2007 John Wiley & Sons, Ltd.

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          Journal
          17902095
          10.1002/jmr.841

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