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      Estudio de inocuidad de Salmonella enterica, subespecie enterica, serotipo enteritidis, var. danysz, lisina negativa en pollos parrilleros

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          Abstract

          Se realizó un estudio con la cepa Salmonella enterica, subespecie enterica, serotipo Enteritidis, var. Danysz, lisina negativa, principio activo de un producto comercial usado para el control biológico de roedores, a fin de evaluar su inocuidad en pollos parrilleros. Se usaron 120 aves de 1 día de edad de ambos sexos de la línea Cobb Vantress divididas en 3 grupos. El grupo A recibió un alimento comercial que contenía 20% del producto raticida al 5o, 6o y 7o día de edad. El grupo B fue inoculado en el buche al 8o día de edad con 108 UFC de la bacteria (1ml) y el grupo C permaneció como control. Se registraron signos clínicos, mortalidad, peso corporal individual por semana, respuesta serológica y la recuperación de la cepa experimental de Salmonella en hígado y bazo. A partir del quinto día del desafío y durante una semana, dos aves por grupo fueron necropsiadas. Adicionalmente, se tomaron muestras de sangre a todas las aves a los 21, 28 y 35 días post desafío y en las aves sacrificadas, para la detección de anticuerpos contra Salmonella mediante la prueba de aglutinación en placa. No se encontraron lesiones macroscópicas compatibles con infección por Salmonella. Los resultados del estudio bacteriológico y serológico fueron negativos a Salmonella sp; sin embargo, un ave a los 28 días y 2 aves a los 35 días post desafío (3/18) del grupo A dieron positivo a la prueba de aglutinación para Salmonella sp. De estas, en una se aisló Salmonella sp. tipificada como lisina positiva. Los pesos corporales a la sétima semana no mostraron diferencias significativas entre grupos.

          Translated abstract

          The Salmonella enterica, serotipe Enteritidis, var Danysz, negative lysine is used as a component in a product for biological control of rodents. The present study evaluated the innocuosness of the strain in 120 1-day old Cobb Vantress broiler chicks of both sexes. They were divided into 3 groups: Group A was fed at 5, 6 and 7 days of age with a commercial broiler feed containing 20% of the rat poison, Group B received 1 ml containing 108 CFU of the bacteria in the crop at 8 days of age, and Group C was kept as a control group. Clinical signs, mortality, recovery of the experimental strain used from liver and spleen, body weight, and serological response were recorded. All birds were weighed every week. Two chicks were ramdomly selected and necropsied from each group on daily basis since the fifth day and for a week. Serum samples and selected tissues were collected for bacteriological culture. Additionally, serum samples were collected at day 21, 28, and 35 after challenge. The bacteriological and serological analysis were negative for Salmonella sp.; however, one bird on day 28 and 2 birds on day 35 after challenge from group A were positive in the agglutination test to Salmonella sp. In one of them, a Salmonella sp. positive lysine was isolated. Body weight differences among groups were not found by the seventh week.

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          Salmonella enterica Serotype enteritidis in Table Egg Layer House Environments and in Mice in U.S. Layer Houses and Associated Risk Factors

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            Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks.

            Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 10(6) colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 10(8) CFU of SE. One group was inoculated with 0.5 ml containing 5 x 10(6) CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 10(8) CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 10(6) and 5 x 10(8) CFU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.
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              Antigenic formulas of the Salmonella serovars

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                Author and article information

                Journal
                rivep
                Revista de Investigaciones Veterinarias del Perú
                Rev. investig. vet. Perú
                Universidad Nacional Mayor de San Marcos. Facultad de Medicina Veterinaria (Lima, , Peru )
                1609-9117
                January 2006
                : 17
                : 1
                : 33-38
                Affiliations
                [02] orgnameUNMSM orgdiv1FMV orgdiv2Laboratorio de Patología Aviar
                [03] orgnameUNMSM orgdiv1FMV orgdiv2Laboratorio de Microbiología y Parasitología Veterinaria
                [04] orgnameUNMSM orgdiv1FMV orgdiv2Laboratorio de Medicina Veterinaria Preventiva
                Article
                S1609-91172006000100006 S1609-9117(06)01700106
                c5aa5936-3d40-4e0e-b653-3f9309e84e38

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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                Figures: 0, Tables: 0, Equations: 0, References: 15, Pages: 6
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                SciELO Peru

                Self URI: Texto completo solamente en formato PDF (ES)
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                Artículos primarios

                rodents,broilers,lysine,Salmonella,broiler,lisina,roedores
                rodents, broilers, lysine, Salmonella, broiler, lisina, roedores

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