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      Impact of Colonoscopy Bowel Preparation on Intestinal Microbiota

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          Abstract

          The gut microbiota is important in maintaining human health, but numerous factors have the potential to alter its composition. Our aim was to examine the impact of a standard bowel preparation on the intestinal microbiota using two different techniques. Fifteen subjects undergoing colonoscopy consumed a bowel preparation comprised of 10 mg bisacodyl and 2 L polyethylene glycol. The microbiota of stool samples, collected one month before, one week before (pre-colonoscopy), and one week, one month, and three to six months after colonoscopy (post-colonoscopy) was evaluated. Two samples were taken three to six months apart from five healthy subjects who did not undergo colonoscopy. Universal primers targeting the V2–V3 region of the 16S rRNA gene were used to PCR amplify all samples for denaturing gradient gel electrophoresis (PCR-DGGE). Pre- and post-colonoscopy samples were compared using Dice’s similarity coefficients. Three samples from ten subjects who underwent colonoscopy, and both samples from the five subjects who didn’t, were used for high-throughput sequencing of the V1–V3 region of the 16S rRNA gene. Samples were curated and analysed in Mothur. Results of the DGGE analyses show that the fecal microbiota of a small number of subjects had short-term changes. High-throughput sequencing results indicated that the variation between the samples of subjects who underwent colonoscopy was no greater than the variation observed between samples from subjects who did not. We conclude that bowel preparation does not have a lasting effect on the composition of the intestinal microbiota for the majority of subjects.

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          Author and article information

          Contributors
          Role: Editor
          Journal
          PLoS One
          PLoS ONE
          plos
          plosone
          PLoS ONE
          Public Library of Science (San Francisco, USA )
          1932-6203
          2013
          1 May 2013
          : 8
          : 5
          Affiliations
          [1 ]Australian National University Medical School, Canberra, Australia
          [2 ]Department of Gastroenterology and Hepatology, Royal Brisbane and Women’s Hospital, Herston, Queensland, Australia
          [3 ]Australian National University Research School of Biology, Canberra, Australia
          [4 ]Gastroenterology and Hepatology Unit, The Canberra Hospital, Canberra, Australia
          University of Waterloo, Canada
          Author notes

          Competing Interests: The authors have declared that no competing interests exist.

          Conceived and designed the experiments: CLO GEA FG PP. Performed the experiments: CLO GEA FG PP. Analyzed the data: CLO GEA FG PP. Contributed reagents/materials/analysis tools: CLO GEA FG PP. Wrote the paper: CLO GEA FG PP.

          Article
          PONE-D-12-32891
          10.1371/journal.pone.0062815
          3641102
          23650530

          This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

          Page count
          Pages: 10
          Funding
          This work was funded by an Australian Post-Graduate Award scholarship. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
          Categories
          Research Article
          Biology
          Anatomy and Physiology
          Digestive System
          Computational Biology
          Sequence Analysis
          Ecology
          Biota
          Genomics
          Metagenomics
          Microbiology
          Medical Microbiology
          Medicine
          Anatomy and Physiology
          Digestive System
          Drugs and Devices
          Gastroenterology and Hepatology
          Colon
          Colonoscopy

          Uncategorized

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