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      Age-Related Changes in Normal and Cataractous Human Lens Crystallins, Separated by Fast-Performance Liquid Chromatography

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      Ophthalmic Research

      S. Karger AG

      Aging, Cataract, Chromatography, Crystallins, Diabetes, Lens

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          The water-soluble crystallins from normal human lenses (n = 32), cataractous lenses of diabetic patients (n = 9) and cataractous lenses of nondiabetic patients (n = 9) were analyzed with fast-performance liquid chromatography. Six different crystallin classes were separated reproducibly by chromatography on Superose 6. The fractions were identified as α<sub>H</sub>, α<sub>L</sub>, β<sub>H</sub>, β<sub>L1</sub>, β<sub>L2 </sub>and low-molecular-weight (LMW) crystallins by their elution order and molecular mass. The results obtained show that during lens aging there is a progressive increase in α<sub>H</sub>-crystallm and a decrease in α<sub>L</sub>-crystallin content, while no significant age-related changes were observed in the LMW fraction. Analysis of changes in crystallin content in human cataractous lenses showed that apparently cataractogenesis can be described as an acceleration of the normal aging process. Important differences were found between the chromatographic profiles of cataracts from diabetic and nondiabetic patients mainly in the LMW fraction, suggesting that in cataract formation of diabetics alternative mechanisms may be superimposed on the normal aging process.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 26
          : 3
          : 149-157
          Center for Ophthalmology, University of Coimbra, Portugal
          267406 Ophthalmic Res 1994;26:149–157
          © 1994 S. Karger AG, Basel

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          Page count
          Pages: 9
          Original Paper

          Vision sciences, Ophthalmology & Optometry, Pathology

          Crystallins, Cataract, Lens, Diabetes, Aging, Chromatography


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