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      Isolation and Characterization of Microsatellite Markers in Brown Planthopper ( Nilaparvata lugens Stål)

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          Abstract

          Brown planthopper ( Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.

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          Strategies for microsatellite isolation: a review.

          L Zane, L Bargelloni, T Patarnello (2002)
          In the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.
          Article 0 comments Cited 311 times – based on 0 reviews     Review now
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            Occurrence of entomopathogenic fungi from agricultural and natural ecosystems in Saltillo, México, and their virulence towards thrips and whiteflies.

            Raul Medina, Jorge San-Juan Lara, Sergio Sánchez-Peña (2011)
            Entomopathogenic fungi were collected from soil in four adjacent habitats (oak forest, agricultural soil, pine reforestation and chaparral habitat) in Saltillo, México using the insect bait method with Tenebrio molitor (L.) (Coleoptera: Tenebrionidae) larvae as bait. Overall, of the larvae exposed to soil, 171 (20%) hosted Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae), 25 (3%) hosted Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and 1 (0.1%) hosted lsaria (=Paecilomyces) sp. (Hypocreales: Cordycipitaceae). B. bassiana was significantly more frequent on larvae exposed to oak forest soil. M. anisopliae was significantly more frequent on larvae exposed to agricultural soil. From the infected bait insects, 93 isolates of B. bassiana and 24 isolates of M. anisopliae were obtained. Strains were tested for their infectivity against Cuban laurel thrips, Gynaikothrips uzeli Zimmerman (Thysanoptera: Phlaeothripidae) and the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). B. bassiana isolates caused the highest mortality on thrips (some causing 88% mortality after 6 days); both fungal species caused similarly high mortality levels against whiteflies (75%) after 6 days. Large amounts of germplasm of entomopathogenic fungi, fundamentally B. bassiana and M. anisopliae, exist in the habitats sampled; pathogenicity varied among strains, and some strains possessed significant virulence. Soils in these habitats are reservoirs of diverse strains with potential for use in biocontrol.
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              Polymorphism revealed by simple sequence repeats

              Wayne Powell, Jim Provan, Gordon C. Machray (1996)
              Article 0 comments Cited 70 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Int J Mol Sci
                Journal ID (iso-abbrev): Int J Mol Sci
                Journal ID (publisher-id): ijms
                Title: International Journal of Molecular Sciences
                Publisher: Molecular Diversity Preservation International (MDPI)
                ISSN (Electronic): 1422-0067
                Publication date Collection: 2012
                Publication date (Electronic): 27 July 2012
                Volume: 13
                Issue: 8
                Pages: 9527-9533
                Affiliations
                State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China; E-Mails: jsl80@ 123456163.com (S.J.); cathychou@ 123456whu.edu.cn (X.Z.); yhj@ 123456whu.edu.cn (H.Y.); bingfangliu@ 123456163.com (B.L.); emilyat10@ 123456126.com (C.Z.); wangshuzhen04@ 123456163.com (S.W.); fred_pxx@ 123456126.com (X.P.); zhulili58@ 123456sina.com (L.Z.); yiding@ 123456whu.edu.cn (Y.D.)
                Author notes
                [* ]Author to whom correspondence should be addressed; E-Mail: gche@ 123456whu.edu.cn ; Tel.: +86-27-6875-2384; Fax: +86-27-6875-2327.
                Article
                Publisher ID: ijms-13-09527
                DOI: 10.3390/ijms13089527
                PMC ID: 3431811
                PubMed ID: 22949813
                SO-VID: ccb2c307-2f23-4882-b1b2-e590c1d0a822
                Copyright © © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
                License:

                This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

                History
                Date revision received : 22 June 2012
                Date accepted : 16 July 2012
                Categories
                Subject: Short Note

                ScienceOpen disciplines: Molecular biology
                Keywords: nilaparvata lugens,microsatellites,genetic diversity,polymorphism
                Data availability:
                ScienceOpen disciplines: Molecular biology
                Keywords: nilaparvata lugens, microsatellites, genetic diversity, polymorphism

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