Retinal projections to the pretectum, accessory optic system and superior colliculus in pigmented and albino ferrets.

Abstract

Retinal projections to the pretectal nuclei, accessory optic system and superior colliculus in pigmented and albino ferrets were studied using anterograde tracing techniques. Both Nissl- and myelin-stained material was used to identify the pretectal nuclei, nuclei of the accessory optic system and the layers of the superior colliculus. Following monocular injection of either horseradish peroxidase or rhodamine-B-isothiocyanate, four pretectal nuclei, including the nucleus of the optic tract, posterior pretectal nucleus, anterior pretectal nucleus and the olivary pretectal nucleus, could be identified to receive direct retinal input in both pigmented and albino strains. In the accessory optic system, retinal terminals were observed in the dorsal, lateral and medial terminal nuclei as well as in the interstitial nucleus of the superior fasciculus, posterior fibres. The retinal projection to the superior colliculus was found to innervate the three superficial layers. The retinal projections to the pretectal nuclei and nuclei of the accessory optic system in the pigmented animals were bilateral, although the label was most dense contralateral to the injected eye. Ipsilateral retinal projections to the pretectal nuclei and nuclei of the accessory optic system appeared to be absent in albino ferrets, i.e. they were invisible with our methods. In both pigmented and albino ferrets retinal terminals in the contralateral superior colliculus densely innervated the three superficial layers. In both strains the ipsilateral projection appeared as clusters which were absent in rostral and caudal poles. In pigmented animals the ipsilateral projection was much denser and more extensive than in albinos. Following injection of retrograde tracers into the brainstem at the level of the dorsal cap of the inferior olive, retrogradely labelled neurons in the pretectum were found in the ipsilateral nucleus of the optic tract. Their somata overlapped mainly with scattered retinal terminals close to the pretectal surface and rarely or not all with the deeper prominent terminal clusters. In the accessory optic system, inferior olive projecting neurons were observed in all four ipsilateral nuclei and fully coincided with the retino-recipient zones. In the superior colliculus, retrogradely labelled neurons were found contralateral to the injection site in the deep layers.