Proteomic techniques in bones forensic samples are increasingly, being applied. The
main aim of forensic sciences is the estimation of postmortem interval. Most current
techniques are useful for the first post-mortem stages. However, in the case of osseous
remains, these techniques may be difficult to use due to the high level of decomposition
of the sample. Our objective was to attempt to know whether there is a protein profile
in human bone remains that would enable a late postmortem. interval ranging from 5
to 20 years postmortem to be estimated. A total of 40 femur bones from 40 different
cadavers (data range 5-20 years) were use. Of the 275 total proteins, we excluded
the circulating ones (n = 227), leaving a total of 48 proteins (29 structural and
19 functional) were found. A multiple correspondence analysis was applied on the 48
proteins. Finally selecting 32 proteins that allowed us to discriminate between the.
two groups of postmortem interval. Analysis of the protein profile present in bone
permits an approximation of the date of death within the studied interval, and could
be used to complement other tests for estimating the postmortem interval.