Anisakid nematodes are important etiological agents for zoonotic human anisakiasis
(or anisakidosis). These parasites in the Turkish waters still remain unexplored.
This study aims the molecular identification of Anisakis species in Turkey's coast
from Black, Aegean and Mediterranean Sea and specifically to screen for zoonotic species
in commonly commercialized a total of 1145 fish belonging to 31 different species
using both polymerase chain reaction-restriction fragment length polymorphism analysis
(PCR-RFLP) and sequencing of the ribosomal internal transcribed spacer (ITS) regions
and the mitochondrial cytochrome C oxidase subunit II (cox2) gene. A total of 776
Anisakis type I larvae were isolated in 56/1145 (4.8%) fish of 7 species from Turkish
waters. The combining all of our results, e.g., morphology, PCR-RFLP, ITS region,
and the cox2 gene, conclusively supported the identification of 3 Anisakis spp. taken
from marine fish hosts, namely Anisakis pegreffii, Anisakis typica and Anisakis simplex
sensu stricto (s.str.)/A. pegreffii hybrid genotype. No Anisakis larvae were isolated
from the Black Sea whereas A. pegreffii, A. typica and A. simplex s.str./A. pegreffii
hybrid genotype was found in the Aegean Sea and A. pegreffii was only isolated from
the Mediterranean Sea. This study represents the first identification of A. typica
and A. simplex s.str./A. pegreffii hybrid genotypes from Turkish waters. Moreover,
in the present study first record of the presence of A. pegreffii is also reported
from Turkish coasts of Aegean and Mediterranean Sea. No zoonotic Anisakis species
were found in commonly commercialized 1025 fish belonging to 16 different species
from the Black Sea, thus Turkish populations who consume captured fish from the Black
Sea may have a less risk of human anisakiasis or allergies. However, the prevalence
of larvae were 47.1% and 46% and recognized zoonotic A. pegreffii were identified
from the Aegean and Mediterranean Sea coast, suggesting a high threat of anisakiasis
or allergies for Turkish populations who consume fish originating in these regions.