For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
Inviting an author to review:
Search for an invisibly decaying $Z^{\prime}$ boson at Belle II in $e^+ e^- \to \mu^+ \mu^- (e^{\pm} \mu^{\mp})$ plus missing energy final states
Find an author and click ‘Invite to review selected article’ near their name.
Search for authorsSearch for similar articles
16
views
31
references
 Top references
cited by
8
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      598
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Chlamydia trachomatis Frequency in a Cohort of HPV-Infected Colombian Women

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Chlamydia trachomatis ( C. trachomatis), an obligate intracellular bacterium, is the commonest infectious bacterial agent of sexual transmission throughout the world. It has been shown that the presence of this bacteria in the cervix represents a risk regarding HPV persistence and, thereafter, in developing cervical cancer (CC). Prevalence rates may vary from 2% to 17% in asymptomatic females, depending on the population being analysed. This study reports the identification of C. trachomatis in a cohort of 219 HPV-infected Colombian females.

          Methods

          C. trachomatis infection frequency was determined during each of the study’s follow-up visits; it was detected by amplifying the cryptic plasmid sequence by polymerase chain reaction (PCR) using two sets of primers: KL5/KL6 and KL1/KL2.

          Infection was defined as a positive PCR result using either set of primers at any time during the study. Cox proportional risk models were used for evaluating the association between the appearance of infection and a group of independent variables.

          Results

          Base line C. trachomatis infection frequency was 28% (n = 61). Most females infected by C. trachomatis were infected by multiple types of HPV (77.42%), greater prevalence occurring in females infected with HPV-16 (19.18%), followed by HPV-58 (17.81%). It was observed that females having had the most sexual partners (HR = 6.44: 1.59–26.05 95%CI) or infection with multiple types of HPV (HR = 2.85: 1.22–6.63 95%CI) had the greatest risk of developing C. trachomatis.

          Conclusions

          The study provides data regarding the epidemiology of C. trachomatis /HPV coinfection in different population groups of Colombian females and contributes towards understanding the natural history of C. trachomatis infection.

          Related collections

          Most cited references31

          • Record: found
          • Abstract: found
          • Article: not found

          Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein.

          C Kreader (1996)
          The benefits of adding bovine serum albumin (BSA) or T4 gene 32 protein (gp32) to PCR were evaluated with reaction mixtures containing substances that inhibit amplification. Whereas 10- to 1,000-fold more FeCl3, hemin, fulvic acids, humic acids, tannic acids, or extracts from feces, freshwater, or marine water were accommodated in PCR when either 400 ng of BSA per microl or 150 ng of gp32 per microl was included in the reactions, neither BSA nor gp32 relieved interference significantly when minimum inhibitory levels of bile salts, bilirubin, EDTA, NaCl, sodium dodecyl sulfate, or Triton X-100 were present. Use of BSA and gp32 together offered no more relief of inhibition than either alone at its optimal level, and neither protein had any noticeable effect on amplification in the absence of inhibitors.
          Article 0 comments Cited 101 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            The natural course of Chlamydia trachomatis infection in asymptomatic Colombian women: a 5-year follow-up study.

            Silvia Franceschi, Chris J L M Meijer, Tineke C. T. M. van der Pouw Kraan … (2005)
            The natural course of Chlamydia trachomatis infection and its risk factors were studied in Colombian women with normal cytological results, during a 5-year period. Eighty-two women who were found to be positive for C. trachomatis at the start of the study were studied at 6-month intervals. At each visit, a cervical scrape sample was obtained for detection of C. trachomatis by use of C. trachomatis endogenous-plasmid polymerase chain reaction (PCR)-enzyme immunoassay and VD2-PCR-reverse line blot assay. Of the women studied, 67% had a single-serovar infection, 10% had a mixed-serovar infection, and 23% had an infection with an unidentified type. An inversed rate of clearance of C. trachomatis infection was observed with oral contraceptive use (hazard ratio [HR], 1.7 [95% confidence interval {CI}, 1.1-2.7]) and first sexual intercourse at >/=20 years of age (HR, 4.3 [95% CI, 2.3-8.0]). Serovars of group B (B, D, and E) and C (H, I, J, and K) had a decreased rate of clearance (rate ratio, 0.4 [95% CI, 0.1-0.9]), compared with that for serovars of the intermediate group (F and G). At 4 years of follow-up, 94% of the women had cleared their infections.
            Article 0 comments Cited 61 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Genital Chlamydia trachomatis infections.

              C. Bébéar, B de Barbeyrac (2009)
              Chlamydia trachomatis infections affect young, sexually active persons. Risk factors include multiple partners and failure to use condoms. The incidence of infection has increased in the past 10 years. Untreated C. trachomatis infections are responsible for a large proportion of salpingitis, ectopic pregnancy, infertility and, to a lesser extent, epididymitis. Screening is a possible intervention to control the infection, which is often asymptomatic. The emergence of lymphogranuloma venereum proctitis in men who have sex with men, in Europe, and of a variant with a deletion in the cryptic plasmid, in Sweden, are new features of C. trachomatis infections in the last years. A diagnosis is best made by using nucleic acid amplification tests, because they perform well and do not require invasive procedures for specimen collection. Single-dose therapy has been a significant development for treatment of an uncomplicated infection of the patient and his or her sexual partner.
              Article 0 comments Cited 60 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Craig Meyers: Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (iso-abbrev): PLoS ONE
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, CA USA )
                ISSN (Electronic): 1932-6203
                Publication date (Electronic): 25 January 2016
                Publication date Collection: 2016
                Volume: 11
                Issue: 1
                Electronic Location Identifier: e0147504
                Affiliations
                [1 ]Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia
                [2 ]Biotechnology Institute, Faculty of Sciences, Universidad Nacional de Colombia, Bogotá, Colombia
                [3 ]Grupo de Investigaciones Microbiológicas–UR (GIMUR), Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Bogotá, Colombia
                [4 ]School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia
                [5 ]School of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia
                Penn State University School of Medicine, UNITED STATES
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Conceived and designed the experiments: EMQC DIRC. Performed the experiments: EMQC DIRC. Analyzed the data: EMQC DIRC SCSDL MC RS LDRO JDR MAP. Wrote the paper: EMQC DIRC SCSDL MC RS LDRO JDR MEP MAP.

                Article
                Publisher ID: PONE-D-15-40045
                DOI: 10.1371/journal.pone.0147504
                PMC ID: 4726460
                PubMed ID: 26807957
                SO-VID: d122b592-8a99-46d6-a415-c9f16a7cd9e7
                Copyright © © 2016 Quinónez-Calvache et al
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 10 September 2015
                Date accepted : 5 January 2016
                Page count
                Figures: 3, Tables: 3, Pages: 14
                Funding
                The authors received no specific funding for this work.
                Categories
                Subject: Research Article
                Subject: Biology and Life Sciences
                Subject: Microbiology
                Subject: Medical Microbiology
                Subject: Microbial Pathogens
                Subject: Bacterial Pathogens
                Subject: Chlamydia Trachomatis
                Subject: Medicine and Health Sciences
                Subject: Pathology and Laboratory Medicine
                Subject: Pathogens
                Subject: Microbial Pathogens
                Subject: Bacterial Pathogens
                Subject: Chlamydia Trachomatis
                Subject: Biology and Life Sciences
                Subject: Organisms
                Subject: Bacteria
                Subject: Chlamydia
                Subject: Chlamydia Trachomatis
                Subject: Medicine and Health Sciences
                Subject: Infectious Diseases
                Subject: Sexually Transmitted Diseases
                Subject: Medicine and Health Sciences
                Subject: Infectious Diseases
                Subject: Sexually Transmitted Diseases
                Subject: Chlamydia Infection
                Subject: Biology and Life Sciences
                Subject: Microbiology
                Subject: Virology
                Subject: Co-Infections
                Subject: Biology and Life Sciences
                Subject: Molecular Biology
                Subject: Molecular Biology Techniques
                Subject: Artificial Gene Amplification and Extension
                Subject: Polymerase Chain Reaction
                Subject: Research and Analysis Methods
                Subject: Molecular Biology Techniques
                Subject: Artificial Gene Amplification and Extension
                Subject: Polymerase Chain Reaction
                Subject: Biology and life sciences
                Subject: Organisms
                Subject: Viruses
                Subject: DNA viruses
                Subject: Papillomaviruses
                Subject: HPV-16
                Subject: Biology and Life Sciences
                Subject: Microbiology
                Subject: Medical Microbiology
                Subject: Microbial Pathogens
                Subject: Viral Pathogens
                Subject: Papillomaviruses
                Subject: HPV-16
                Subject: Medicine and Health Sciences
                Subject: Pathology and Laboratory Medicine
                Subject: Pathogens
                Subject: Microbial Pathogens
                Subject: Viral Pathogens
                Subject: Papillomaviruses
                Subject: HPV-16
                Subject: Biology and Life Sciences
                Subject: Organisms
                Subject: Viruses
                Subject: Viral Pathogens
                Subject: Papillomaviruses
                Subject: HPV-16
                Subject: Medicine and Health Sciences
                Subject: Urology
                Subject: Genitourinary Infections
                Subject: Human Papillomavirus Infection
                Subject: Medicine and Health Sciences
                Subject: Infectious Diseases
                Subject: Sexually Transmitted Diseases
                Subject: Human Papillomavirus Infection
                Subject: Medicine and Health Sciences
                Subject: Infectious Diseases
                Subject: Viral Diseases
                Subject: Human Papillomavirus Infection
                Subject: People and places
                Subject: Geographical locations
                Subject: South America
                Subject: Colombia
                Custom metadata
                Data Availability All relevant data are within the paper.

                ScienceOpen disciplines: Uncategorized
                Data availability:
                ScienceOpen disciplines: Uncategorized

                Comments

                Comment on this article

                scite_

                Similar content598

                See all similar

                Cited by7

                See all cited by

                Most referenced authors470

                See all reference authors