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      The deubiquitinating enzyme activity of USP22 is necessary for regulating HeLa cell growth.

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          Abstract

          Ubiquitin-specific protease 22 (USP22) can regulate the cell cycle and apoptosis in many cancer cell types, while it is still unclear whether the deubiquitinating enzyme activity of USP22 is necessary for these processes. As little is known about the impact of USP22 on the growth of HeLa cell, we observed whether USP22 can effectively regulate HeLa cell growth as well as the necessity of deubiquitinating enzyme activity for these processes in HeLa cell. In this study, we demonstrate that USP22 can regulate cell cycle but not apoptosis in HeLa cell. The deubiquitinating enzyme activity of USP22 is necessary for this process as confirmed by an activity-deleted mutant (C185S) and an activity-decreased mutant (Y513C). In addition, the deubiquitinating enzyme activity of USP22 is related to the levels of BMI-1, c-Myc, cyclin D2 and p53. Our findings indicate that the deubiquitinating enzyme activity of USP22 is necessary for regulating HeLa cell growth, and it promotes cell proliferation via the c-Myc/cyclin D2, BMI-1 and p53 pathways in HeLa cell.

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          Author and article information

          Journal
          Gene
          Gene
          Elsevier BV
          1879-0038
          0378-1119
          Nov 01 2015
          : 572
          : 1
          Affiliations
          [1 ] Department of Epidemiology and Statistics, School of Public Health, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, China.
          [2 ] Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, China.
          [3 ] Department of Epidemiology and Statistics, School of Public Health, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, China. Electronic address: tianqingbao1980@aliyun.com.
          Article
          S0378-1119(15)00799-4
          10.1016/j.gene.2015.06.075
          26143114
          d5e5bd70-fc1b-4073-a813-557bd193d78c
          History

          Apoptosis,Cell cycle,Deubiquitinating enzyme activity,HeLa cell,Small interfering RNA,USP22

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