7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Degranulation and cytokine expression in human cord blood-derived mast cells cultured in serum-free medium with recombinant human stem cell factor.

      Molecules and Cells
      Cell Degranulation, drug effects, physiology, Cells, Cultured, Chymases, Culture Media, Serum-Free, chemistry, Cyclooxygenase 2, Cytokines, biosynthesis, immunology, Female, Fetal Blood, cytology, Humans, Interleukin-6, pharmacology, Isoenzymes, Mast Cells, secretion, Membrane Proteins, Pertussis Toxin, Pregnancy, Prostaglandin-Endoperoxide Synthases, Recombinant Proteins, Serine Endopeptidases, Stem Cell Factor, Substance P, beta-N-Acetylhexosaminidases, p-Methoxy-N-methylphenethylamine

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Human cord blood-derived mast cells (HCMC) grown in medium with serum and recombinant human stem cell factor (rhSCF) with or without interleukin (IL)-6 are less mature than human skin mast cells (HSMC). We found that c-kit-positive HCMC cultured for 8-10 weeks with rhSCF in serum-free medium became sensitive to basic secretatogues and expressed the serine protease, chymase, which is preferentially expressed in HSMC. The HCMC release beta-hexosaminidase (beta-HEX) within 1 min of stimulation with compound 48/80 or substance P, and release was suppressed by pertussis toxin. Approximately 34% of the HCMC in the serum-free culture stained positively with chymase antibody. Chymase and c-kit levels, and responsiveness to basic secretagogues, increased substantially after an additional 2 weeks in a serum-free environment with rhIL-6 and rhSCF. Moreover, Fc(epsilon)RI-dependent activation of the HCMC resulted in induction of cytokines and cyclooxygenase-2. These results show that HCMC can differentiate into a phenotype morphologically and functionally similar to HSMC if exposed to SCF in serum-free medium.

          Related collections

          Author and article information

          Comments

          Comment on this article