- Record: found
- Abstract: not found
- Article: not found
The hemagglutinin–neuraminidase protein of peste des petits ruminants virus is biologically active when transiently expressed in mammalian cells
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
The genes coding for the surface glycoproteins hemagglutinin-neuraminidase (HN) of
the peste des petits ruminants virus (PPRV) and hemagglutinin (H) of rinderpest virus
(RPV) were cloned in a cytomagalovirus promoter driven expression vector and expressed
transiently in mammalian cells. The protein expression was apparent 24 h after transfection
and the expressed proteins were detected at the cell surface. The transiently expressed
PPRV HN protein was found to be biologically active in possessing hemadsorption and
neuraminidase activities. On the other hand, RPV H protein exhibited neuraminidase
activity but was deficient in hemadsorption activity. The substrate specificity of
the neuraminidase activity of these two proteins differed distinctly. The presence
of neuraminidase activity in both PPRV HN and RPV H proteins is unusual among members
of the morbillivirus genus.