Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Premature ageing in mice expressing defective mitochondrial DNA polymerase.

Nature

genetics, Adipose Tissue, Aging, Premature, complications, enzymology, pathology, Alopecia, Animals, Body Composition, Body Weight, Bone Density, DNA Mutational Analysis, DNA, Mitochondrial, DNA-Directed DNA Polymerase, metabolism, Homozygote, Kyphosis, Mice, Mitochondria, Mutagenesis, Mutation, Phenotype, Transgenes

Read this article at

ScienceOpenPublisherPubMed
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      Point mutations and deletions of mitochondrial DNA (mtDNA) accumulate in a variety of tissues during ageing in humans, monkeys and rodents. These mutations are unevenly distributed and can accumulate clonally in certain cells, causing a mosaic pattern of respiratory chain deficiency in tissues such as heart, skeletal muscle and brain. In terms of the ageing process, their possible causative effects have been intensely debated because of their low abundance and purely correlative connection with ageing. We have now addressed this question experimentally by creating homozygous knock-in mice that express a proof-reading-deficient version of PolgA, the nucleus-encoded catalytic subunit of mtDNA polymerase. Here we show that the knock-in mice develop an mtDNA mutator phenotype with a threefold to fivefold increase in the levels of point mutations, as well as increased amounts of deleted mtDNA. This increase in somatic mtDNA mutations is associated with reduced lifespan and premature onset of ageing-related phenotypes such as weight loss, reduced subcutaneous fat, alopecia (hair loss), kyphosis (curvature of the spine), osteoporosis, anaemia, reduced fertility and heart enlargement. Our results thus provide a causative link between mtDNA mutations and ageing phenotypes in mammals.

      Related collections

      Author and article information

      Journal
      10.1038/nature02517
      15164064

      Comments

      Comment on this article