Record: found
Abstract: found
Article: not found

Presence of immunoreactive endothelin in human plasma.

Author(s): Y Hirata, T. Emori, K Ando, M Shichiri, F Marumo

Publication date: 1989-03-13

Keywords: Adult, Chromatography, High Pressure Liquid, Endothelins, Endothelium, Vascular, Female, Humans, Male, Peptides, blood, Radioimmunoassay, Reference Values

Read this article at

ScienceOpenPubMed

Bookmark

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.