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      Evaluation of the Antibacterial Efficacy of QMix and AgNP Solutions in Root Canals of Primary Molars: An In-Vitro Study

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          Abstract

          Purpose

          The study aimed to evaluate QMix 2in1 and silver nanoparticles (AgNPs) solution in eliminating Enterococcus faecalis ( Efaecalis) bacteria within root canals of primary molars.

          Materials and methods

          The study sample consisted of 45 extracted primary molars, which were divided into three groups: Group 1 (control) NaOCl 5.25%, Group 2 (experimental) QMix 2 in 1, and Group 3 (experimental) AgNPs 4000 ppm. The root canals were sterilized within an autoclave and then contaminated with Efaecalis bacteria. The root canals were irrigated for five minutes in a quantity of 3 ml using a 31-gauge irrigation needle, and then bacterial smears were taken.

          Results

          Sodium hypochlorite, AgNPs, and QMix 2in1 effectively reduced the bacterial count of Enterococcus within root canals of primary molars. There were statistical differences between all groups. QMix 2in1 solution showed the greatest antibacterial efficacy, then NaOCl solution and AgNPs solution. 

          Conclusion

          All irrigation solutions used are effective in eliminating Efaecalis. QMix 2in1 can be considered a good alternative to sodium hypochlorite in irrigation root canals of primary teeth.

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          Most cited references30

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          Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli.

          The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log(10) CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.
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            Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria.

            In the present scenario, pharmaceutical and biomedical sectors are facing the challenges of continuous increase in the multidrug-resistant (MDR) human pathogenic microbes. Re-emergence of MDR microbes is facilitated by drug and/or antibiotic resistance, which is acquired way of microbes for their survival and multiplication in uncomfortable environments. MDR bacterial infections lead to significant increase in mortality, morbidity and cost of prolonged treatments. Therefore, development, modification or searching the antimicrobial compounds having bactericidal potential against MDR bacteria is a priority area of research. Silver in the form of various compounds and bhasmas have been used in Ayurveda to treat several bacterial infections since time immemorial. As several pathogenic bacteria are developing antibiotic resistance, silver nanoparticles are the new hope to treat them. This review discusses the bactericidal potential of silver nanoparticles against the MDR bacteria. This multiactional nanoweapon can be used for the treatment and prevention of drug-resistant microbes. © 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
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              Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm.

              The purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (AgNPs) as an irrigant or medicament against Enterococcus faecalis biofilms formed on root dentin. Dentin sections were inoculated with E. faecalis for 4 weeks to establish a standard monospecies biofilm model. These biofilms were tested in 2 stages. In stage 1, the biofilms were irrigated with 0.1% AgNP solution, 2% sodium hypochlorite, and sterile saline for 2 minutes, respectively. In stage 2, the biofilms were treated with AgNP gel (0.02% and 0.01%) and calcium hydroxide for 7 days. The ultrastructure of one half of the specimens from each group was evaluated by using scanning electron microscopy, whereas the structure and distribution of viable bacteria of the other half of the specimens were assessed with confocal laser scanning microscopy combined with viability staining. Syringe irrigation with 0.1% AgNP solution did not disrupt the biofilm structure, and the proportion of viable bacteria in the biofilm structures was not different from that of the saline group (P > .05) but was less than that of the control group (P < .05). The biofilms treated with 0.02% AgNP gel as medicament significantly disrupted the structural integrity of the biofilm and resulted in the least number of post-treatment residual viable E. faecalis cells compared with 0.01% AgNP gel and calcium hydroxide groups (P < .05). The findings from this study suggested that the antibiofilm efficacy of AgNPs depends on the mode of application. AgNPs as a medicament and not as an irrigant showed potential to eliminate residual bacterial biofilms during root canal disinfection. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
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                Author and article information

                Journal
                Cureus
                Cureus
                2168-8184
                Cureus
                Cureus (Palo Alto (CA) )
                2168-8184
                7 September 2022
                September 2022
                : 14
                : 9
                : e28877
                Affiliations
                [1 ] Department of Pediatric Dentistry, Damascus University, Damascus, SYR
                [2 ] Department of Pedodontics and Preventive Dentistry, Damascus University, Damascus, SYR
                [3 ] Department of Chemistry and Biochemistry, Damascus University, Damascus, SYR
                [4 ] Department of Endodontics, Damascus University, Damascus, SYR
                Author notes
                Article
                10.7759/cureus.28877
                9540512
                df3676b5-81a8-4692-adf1-12ab811bdcf0
                Copyright © 2022, Alkhourbotly et al.

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 7 September 2022
                Categories
                Dentistry

                primary molars,enterococcus faecalis,antibacterial efficacy,sodium hypochlorite,qmix,irrigation

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