Hormetic heat stress and HSF-1 induce autophagy to improve survival and proteostasis in C. elegans

Abstract

Stress-response pathways have evolved to maintain cellular homeostasis and to ensure the survival of organisms under changing environmental conditions. Whereas severe stress is detrimental, mild stress can be beneficial for health and survival, known as hormesis. Although the universally conserved heat-shock response regulated by transcription factor HSF-1 has been implicated as an effector mechanism, the role and possible interplay with other cellular processes, such as autophagy, remains poorly understood. Here we show that autophagy is induced in multiple tissues of Caenorhabditis elegans following hormetic heat stress or HSF-1 overexpression. Autophagy-related genes are required for the thermoresistance and longevity of animals exposed to hormetic heat shock or HSF-1 overexpression. Hormetic heat shock also reduces the progressive accumulation of PolyQ aggregates in an autophagy-dependent manner. These findings demonstrate that autophagy contributes to stress resistance and hormesis, and reveal a requirement for autophagy in HSF-1-regulated functions in the heat-shock response, proteostasis and ageing.

Abstract

Mild heat stress has beneficial effects on organismal health and survival. Here, Kumsta et al. show that a mild heat shock and HSF-1 overexpression induce autophagy in multiple tissues of C. elegans and autophagy-related genes are essential for both heat shock-induced and HSF-1–mediated stress resistance and longevity.