Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells

Summary

The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are largely unknown. We established persistent CVB infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled infection-induced changes in cellular protein expression and secretion using mass spectrometry-based proteomics. Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of antiviral immune response proteins, and also several other proteins, differed clearly between the two persistent infections. Our results provide extensive information about the protein-level changes induced by persistent CVB infection and the potential virus-associated variability in the outcomes of these infections.

Graphical Abstract

Highlights

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  Persistent CVB infection in PANC-1 cells established using two CVB1 strains

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  Infections influenced host protein expression and secretion broadly

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  Changes in, e.g., mitochondria, virus receptors, and regulated secretory pathway

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  Persistency-triggered antiviral immune responses differed between the virus strains

Abstract

Biological Sciences; Microbiology; Virology; Proteomics