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      Peppermint essential oil as an anesthetic for and toxicity to juvenile silver catfish Translated title: Óleo essencial de hortelã-pimenta como anestésico e sua toxicidade para juvenis de jundiá

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          Abstract

          Abstract: The objective of this work was to evaluate peppermint (Mentha piperita) essential oil toxicity to and potential as an anesthetic for juvenile silver catfish (Rhamdia quelen). To determine the lethal concentration at 4 hours (LC50-4h), 210 fish (3.08±0.8 g and 7.59±0.67 cm) were exposed to 0, 20, 50, 80, 110, and 140 mg L-1 essential oil. To evaluate the anesthetic potential, nine fish were individually exposed to each oil concentration (50, 80, 110, and 140 mg L-1) used. Water quality parameters were controlled. The mortality rate and the severity and extent of the gill injuries of silver catfish juveniles at 4 hours of exposure increased with increasing peppermint essential oil concentrations, with LC50-4h estimated to be 75.06 mg L-1. The main gill injuries were: congestion of the venous sinus of the primary lamella and at the base of the secondary lamella; interlamellar hyperplasia with fusion of lamellae; epithelial detachment; dilation of the venous sinus; edema adjacent to the lamella; and aneurysm. However, this oil is an effective anesthetic for silver catfish juveniles at the concentration of 80 mg L-1, with a short time of anesthesia (<4 min) and recovery (<10 min), with no mortality.

          Translated abstract

          Resumo: O objetivo deste trabalho foi avaliar a toxicidade e o potencial como anestésico do óleo essencial de hortelã-pimenta (Mentha piperita) para juvenis de jundiá (Rhamdia quelen). Para determinar a concentração letal em 4 horas (CL50-4h), 210 peixes (3,08±0,8 g e 7,59±0,67 cm) foram expostos a 0, 20, 50, 80, 110 e 140 mg L-1 do óleo essencial. Para avaliar o potencial anestésico, nove peixes foram individualmente expostos à cada concentração (50, 80, 110 e 140 mg L-1) de óleo utilizada. Os parâmetros de qualidade da água foram controlados. A taxa de mortalidade e a severidade e a abrangência das lesões branquiais de juvenis de jundiá em 4 horas de exposição cresceu com o aumento da concentração do óleo essencial de hortelã-pimenta, com CL50-4h estimada em 75,06 mg L-1. As principais lesões branquiais foram: congestão do seio venoso da lamela primária e na base da lamela secundária; hiperplasia interlamelar e com fusão das lamelas; descolamento do epitélio; dilatação do seio venoso; edema justalamelar; e aneurisma. Entretanto, esse óleo é eficiente anestésico para juvenis de jundiá na concentração de 80 mg L-1, com reduzido tempo de anestesia (<4 min) e de recuperação (<10 min), sem mortalidade.

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          Most cited references22

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          Histopathology in fish: proposal for a protocol to assess aquatic pollution

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            Antimicrobial screening of Mentha piperita essential oils.

            Essential oils of peppermint Mentha piperita L. (Lamiaceae), which are used in flavors, fragrances, and pharmaceuticals, were investigated for their antimicrobial properties against 21 human and plant pathogenic microorganisms. The bioactivity of the oils menthol and menthone was compared using the combination of in vitro techniques such as microdilution, agar diffusion, and bioautography. It was shown that all of the peppermint oils screened strongly inhibited plant pathogenic microorganisms, whereas human pathogens were only moderately inhibited. Chemical compositions of the oils were analyzed by GC and GC/MS. Using the bioautography assay, menthol was found to be responsible for the antimicrobial activity of these oils.
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              Essential oils of Lippia sidoides and Mentha piperita against monogenean parasites and their influence on the hematology of Nile tilapia

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                pab
                Pesquisa Agropecuária Brasileira
                Pesq. agropec. bras.
                Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira (Brasília, DF, Brazil )
                0100-204X
                1678-3921
                2019
                : 54
                : e00367
                Affiliations
                [3] Manaus Amazonas orgnameEmbrapa Amazônia Ocidental Brazil edsandra.chagas@ 123456embrapa.br
                [2] Florianópolis Santa Catarina orgnameUniversidade Federal de Santa Catarina orgdiv1Laboratório de Biologia e Cultivo de Peixes de Água Doce Brazil emiliaspanghero@ 123456hotmail.com
                [1] Penedo orgnameUniversidade Federal de Alagoas orgdiv1Laboratório de Inovação em Aquacultura Brazil diogo.spanghero@ 123456penedo.ufal.br
                Article
                S0100-204X2019000101102
                10.1590/s1678-3921.pab2019.v54.00367
                e9412ab4-2995-4a9c-96a6-cab4a1488788

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 23 January 2019
                : 08 December 2017
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 31, Pages: 0
                Product

                SciELO Brazil

                Categories
                Aquaculture

                Mentha piperita,concentração letal,histologia,brânquias,anestesia,Rhamdia quelen,lethal concentration,histology,gill,anesthesia

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