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      Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells.

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          Abstract

          Differentiation of embryonic stem (ES) cells from a pluripotent to a committed state involves global changes in genome expression patterns. Gene activity is critically determined by chromatin structure and interactions of chromatin binding proteins. Here, we show that major architectural chromatin proteins are hyperdynamic and bind loosely to chromatin in ES cells. Upon differentiation, the hyperdynamic proteins become immobilized on chromatin. Hyperdynamic binding is a property of pluripotent cells, but not of undifferentiated cells that are already lineage committed. ES cells lacking the nucleosome assembly factor HirA exhibit elevated levels of unbound histones, and formation of embryoid bodies is accelerated. In contrast, ES cells, in which the dynamic exchange of H1 is restricted, display differentiation arrest. We suggest that hyperdynamic binding of structural chromatin proteins is a functionally important hallmark of pluripotent ES cells that contributes to the maintenance of plasticity in undifferentiated ES cells and to establishing higher-order chromatin structure.

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          Author and article information

          Journal
          Dev Cell
          Developmental cell
          Elsevier BV
          1534-5807
          1534-5807
          Jan 2006
          : 10
          : 1
          Affiliations
          [1 ] National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
          Article
          S1534-5807(05)00421-1 NIHMS18673
          10.1016/j.devcel.2005.10.017
          1868458
          16399082
          eabfb6a2-a864-4398-8b07-9c65f6c327a4
          History

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