<i>Slc25a5</i> regulates adipogenesis by modulating ERK signaling in OP9 cells

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Abstract

Background

A comprehensive understanding of the molecular mechanisms of adipogenesis is a critically important strategy for identifying new targets for obesity intervention.

Methods

Transcriptomic and lipidomic approaches were used to explore the functional genes regulating adipogenic differentiation and their potential mechanism of action in OP9 cells and adipose-derived stem cells. Oil Red O staining was used to detect oil droplets in adipocytes.

Results

RNA sequencing (RNA-seq) showed that Slc25a5 expression was significantly upregulated in adipogenic differentiation. Depletion of Slc25a5 led to the suppressed expression of adipogenesis-related genes, reduced the accumulation of triglycerides, and inhibited PPARγ protein expression. Moreover, the knockdown of Slc25a5 resulted in significant reduction of oxidative phosphorylation (OXPHOS) protein expression (ATP5A1, CQCRC2, and MTCO1) and ATP production. The RNA-seq and real-time quantitative polymerase chain reaction (RT–qPCR) results suggested that adipogenic differentiation is possibly mediated by ERK1/2 phosphorylation, and this hypothesis was confirmed by intervention with PD98059 (an ERK 1/2 inhibitor).

Conclusions

This study indicates that Slc25a5 inhibits adipogenesis and might be a new therapeutic target for the treatment of obesity.

Supplementary Information

The online version contains supplementary material available at 10.1186/s11658-022-00314-y.

Related collections

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Author and article information

Contributors

Yong Q. Chen: yqc_lab@126.com , yqchen@jiangnan.edu.cn

Journal

Journal ID (nlm-ta): Cell Mol Biol Lett

Journal ID (iso-abbrev): Cell Mol Biol Lett

Title: Cellular & Molecular Biology Letters

Publisher: BioMed Central (London )

ISSN (Print): 1425-8153

ISSN (Electronic): 1689-1392

Publication date (Electronic): 2 February 2022

Publication date PMC-release: 2 February 2022

Publication date Collection: 2022

Volume: 27

Electronic Location Identifier: 11

Affiliations

[1 ]GRID grid.258151.a, ISNI 0000 0001 0708 1323, Wuxi School of Medicine, , Jiangnan University, ; 1800 Lihu Road, Wuxi, 214122 Jiangsu China

[2 ]Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, China

[3 ]GRID grid.258151.a, ISNI 0000 0001 0708 1323, School of Food Science and Technology, , Jiangnan University, ; Wuxi, China

Article

Publisher ID: 314

DOI: 10.1186/s11658-022-00314-y

PMC ID: 8903613

PubMed ID: 35109789

SO-VID: ee7e4446-c61f-4fd4-a4f3-4c18d8061b1c

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 7 October 2021

Date accepted : 17 January 2022

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 31771539

Award ID: 82000808

Award Recipient : Shenglong Zhu Yong Q. Chen

Funded by: Innovation and Application Project of Medical and Public Health Technology of Wuxi Science and Technology

Award ID: N20202005