Allogeneic haematopoietic stem cell transplantation from SARS-CoV-2 positive donors

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of COVID-19, but up to half of people infected with the virus are asymptomatic. 1 Moreover, SARS-CoV-2 RNAemia might occur in about 15% of symptomatic patients but also in a minority of those who are asymptomatic, 2 making possible—yet unconfirmed to date—the possibility of viral transmission through blood products,3, 4 in particular in the setting of allogeneic haematopoietic stem cell transplantation (HSCT). Here we report two cases of patients with acute myeloid leukaemia from our hospital who were transplanted in early October, 2020, with peripheral blood stem cells harvested from donors who tested positive for SARS-CoV-2 by RT-PCR on nasopharyngeal swabs at the time of cell collection. Patient one is aged 60 years and reached first complete remission of acute myeloid leukaemia with myelodysplasia-related changes after induction chemotherapy. The patient then received consolidation with intermediate-dose cytarabine and was directed to haploidentical HSCT after reduced-intensity conditioning, using post-transplantation cyclophosphamide as prophylaxis for graft versus host disease. The donor was the patient's 22-year-old child. Patient two is aged 64 years and reached first complete remission of a RUNX1-mutated acute myeloid leukaemia after induction chemotherapy. After receiving two consolidation courses with intermediate-dose cytarabine, a relapse occurred with profound pancytopenia and a marrow blast level of 13%. Meanwhile, the patient developed pulmonary invasive fungal infections and was referred for HSCT from a 50-year-old sibling donor. In both cases, the donors tested negative for SARS-CoV-2 by RT-PCR on nasopharyngeal swabs 8 days before transplantation. However, at time of cell collection, both donors were tested again and found to be positive although still asymptomatic. Acute infection was found in the donor for patient two on the basis of seroconversion for anti-nucleoprotein and a significant increase in total anti-S1 antibodies (appendix). 5 With regards to the benefit–risk balance for each recipient, recipients received their transplantations on the same day as collection, with recipients being tested twice a week for SARS-CoV-2 on nasaopharyngeal swabs and plasma. All tests remained negative over a 4-week follow-up period. Both recipients developed fever at some point after transplantation, without other symptoms suggesting COVID-19 illness. The COVID-19 pandemic has created substantial barriers to timely donor assessment, cell collection, and graft transport. In the 14 days before donation, donors should practice good hygiene and isolate themselves as much as feasible during this period. Unnecessary travel should be avoided. Some guidelines recommend that donors should be tested for SARS-CoV-2 so that results are available before their admission for the collection procedure. 6 Stem cell products can be frozen at the collection site or recipient-treating centre if substantial transport delays related to travel restrictions are likely. Indeed, cryopreservation of stem cell products is a secure approach in the pandemic context, preventing unexpected and undesirable events, such as transport delays or last-minute disqualification of a donor who is symptomatic and SARS-CoV-2 positive on the day of collection, that could preclude timely delivery of the graft to a patient who has already received the pre-transplantation conditioning regimen. Using this logistical organisation, cryopreserved grafts will be expected to be received at the recipient-treating centre before the start of conditioning. In parallel, cryopreservation might be used with the aim to apply a formal post-donation so-called cryo-quarantine period whereby a donation will only qualify for release if the donor tests negative or remains symptom-free at the end of the cryo-quarantine period. Although theoretically decreasing the risk of viral transmission from asymptomatic donors to recipients, systematically applying such a cryo-quarantine period remains debatable. First, as highlighted in the World Marrow Donor Association recommendations (updated as of Dec 8, 2020), failure to qualify for release can occur as a result of COVID-19 exposure that occurs after collection. Second, in the absence of symptoms, a positive nasopharyngeal swab on day 14 after collection or later is not consistent with the presence of pre-symptomatic SARS-CoV-2 infection at the time of collection. Third, cryopreservation for a minimum quarantine of 2 weeks might be directly harmful due to the urgent need for the transplantation in patients with aggressive, hard-to-control, underlying diseases such as acute leukaemia. In non-malignant diseases, such as severe aplastic anaemia, fresh cells rather than frozen cells have been found to be better for blood and marrow transplantation, in correlation with graft cell loss induced by the cryopreservation process. 7 Fourth, the two cases we reported here suggest that, despite the absence of pathogen-reduction treatment as is used for transfusion products, peripheral blood stem cells that have been harvested from donors who are SARS-CoV-2 positive might not lead to haematogenous viral transmission. Notably, this absence of transmission has been previously reported in a paediatric patient who received a bone marrow transplantation harvested from a sibling donor who tested positive for SARS-CoV-2 by RT-PCR on nasopharyngeal swabs although they were asymptomatic. 8 Finally, independent of risk to patient, strong ethical issues also exist regarding cryopreservation of donor stem cell products, particularly in the setting of voluntary unrelated donors. Cryopreservation results in a substantial increase of non-transfused unrelated donor stem cell products, possibly related to progression of the underlying malignancy in the recipient or acquisition of additional comorbidities during the quarantine period. 9 Beyond the possibility of blood or HSCT transmission risk, important public health considerations and a variety of community measures around the world are having a major effect on HSCT donors and collection facilities. In this rapidly evolving pandemic, the situation in many countries is likely to change quickly in the coming months and years. The transplantation community has to remain vigilant to the evolving definition of risk-adapted procedures, taking advantage of the accumulation of knowledge in the field. © 2021 Garo/Phanie/Science Photo Library 2021 Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.