The Deficits in Spatial Visualization may affect the performance of 3D Computer Animation among Multimedia undergraduates. Spatial Visualization can be observed as a unique type of intelligence distinguishable from other forms of intelligence, such as verbal ability and reasoning ability. Most students experienced difficulties in performing the complicated visualization task for creating 3D animation. Students whose spatial visualization skills are insufficient to complete the assignment will not be able to obtain good grades in the 3D computer animation course. The purpose of this research is to explore the relationship between Spatial Visualization, gender, and 3D Computer Animation performance amongst undergraduates.