Draft Genome Sequence of  Enterobacter asburiae  UFMG-H9, Isolated from Urine from a Healthy Bovine Heifer (Gyr Breed)

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Abstract

Enterobacter asburiae is part of the Enterobacter cloacae complex, related to nosocomial opportunistic infections in humans. Here, we report the draft genome of E. asburiae strain UFMG-H9, an isolate from urine from a healthy Gyr heifer.

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Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.

J. R. Marchesi, T Sato, A. Weightman … (1998)

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

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Emergence and Spread of Carbapenem-Resistant and Aminoglycoside-Panresistant Enterobacter cloacae Complex Isolates Coproducing NDM-Type Metallo-β-Lactamase and 16S rRNA Methylase in Myanmar

Satoshi Oshiro, Tatsuya Tada, Shin Watanabe … (2020)

The emergence of multidrug-resistant E. cloacae complex has become a public health threat worldwide. E. xiangfangensis is a recently classified species belonging to E. cloacae complex. Here, we report a clonal dissemination of multidrug-resistant E. xiangfangensis ST200 producing two types of New Delhi metallo-β-lactamase (NDM-type MBL), NDM-1 and -4, and three types of 16S rRNA methylases, ArmA, RmtC, and RmtE, in hospitals in Myanmar. The observation of these multidrug-resistant E. xiangfangensis ST200 isolates stresses the urgency to continue molecular epidemiological surveillance of these pathogens in Myanmar and in South Asian countries.

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Antibiotic Resistance of Enterobacteriaceae Isolated from Fresh Fruits and Vegetables and Characterization of their AmpC β-Lactamases.

Zahra Al-Kharousi, Nejib Guizani, Abdullah M. Al-Sadi … (2019)

Enterobacteria may gain antibiotic resistance and be potent pathogens wherever they are present, including in fresh fruits and vegetables. This study tested the antibiotic resistance of enterobacteria isolated from 13 types of local and imported fresh fruits and vegetables (n = 105), using the standard Kirby-Bauer disk diffusion method. Phenotypic and genotypic characterizations of AmpC β-lactamases were determined in cefoxitin-resistant isolates. Ten percent of the enterobacteria tested (n = 88) were pansusceptible, 74% were resistant to at least one antibiotic, and 16% were multidrug resistant. Enterobacteria isolates showed the highest antibiotic resistance against ampicillin (66%), cephalothin (57%), amoxicillin-clavulanic acid (33%), cefoxitin (31%), tetracycline (9%), nalidixic acid (7%), trimethoprim (6%), and kanamycin (5%). Three isolates showed intermediate resistance to the clinically important antibiotic imipenem. Escherichia coli isolated from lettuce exhibited multidrug resistance against five antibiotics. Fifteen isolates were confirmed to have AmpC β-lactamase, using the inhibitor-based test and the antagonism test; the latter test confirmed that the enzyme was an inducible type. Four types of ampC β-lactamase genes (CIT, EBC, FOX, and MOX) were detected in eight isolates: four Enterobacter cloacae isolates and one isolate each of Citrobacter freundii, Enterobacter asburiae, Enterobacter hormaechei, and Enterobacter ludwigii. It was concluded that fresh fruits and vegetables might play a role as a source or vehicle for transferring antibiotic-resistant bacteria that might spread to other countries through exportation. The clinically significant AmpC β-lactamase was rarely documented in the literature on bacteria isolated from fruits and vegetables, and to our knowledge, this is the first report on the detection of an inducible type in such commodities.

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Contributors

Christina A. Cuomo: Role: Editor

Journal

Journal ID (nlm-ta): Microbiol Resour Announc

Journal ID (iso-abbrev): Microbiol Resour Announc

Journal ID (hwp): ga

Journal ID (pmc): mra

Journal ID (publisher-id): MRA

Title: Microbiology Resource Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2576-098X

Publication date (Electronic): 21 May 2020

Publication date Collection: May 2020

Volume: 9

Issue: 21

Electronic Location Identifier: e00385-20

Affiliations

[a ]Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil

[b ]Bioinformatics Program, Loyola University Chicago, Chicago, Illinois, USA

[c ]Empresa de Pesquisa Agropecuária de Minas Gerais, Uberaba, MG, Brazil

[d ]Department of Biology, Loyola University Chicago, Chicago, Illinois, USA

[e ]Department of Computer Science, Loyola University Chicago, Chicago, Illinois, USA

[f ]Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA

Broad Institute

Author notes

Address correspondence to Catherine Putonti, cputonti@ 123456luc.edu .

Citation Giannattasio-Ferraz S, Maskeri L, Oliveira AP, Barbosa-Stancioli EF, Putonti C. 2020. Draft genome sequence of Enterobacter asburiae UFMG-H9, isolated from urine from a healthy bovine heifer (Gyr breed). Microbiol Resour Announc 9:e00385-20. https://doi.org/10.1128/MRA.00385-20.

Author information

Catherine Putonti https://orcid.org/0000-0003-3049-5991

Article

Publisher ID: MRA00385-20

DOI: 10.1128/MRA.00385-20

PMC ID: 7242673

PubMed ID: 32439671

SO-VID: f0bc33d3-c3f9-445f-aeab-833bd7bff111

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 12 April 2020

Date accepted : 4 May 2020

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Figures: 0, Tables: 1, Equations: 0, References: 7, Pages: 2, Words: 1222

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cover-date May 2020

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Authors: Silvia Giannattasio-Ferraz, Adriana Ene, Vitor Júnio Gomes …

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Draft Genome Sequence of Enterobacter asburiae UFMG-H9, Isolated from Urine from a Healthy Bovine Heifer (Gyr Breed)

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Most cited references 4

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.

Emergence and Spread of Carbapenem-Resistant and Aminoglycoside-Panresistant Enterobacter cloacae Complex Isolates Coproducing NDM-Type Metallo-β-Lactamase and 16S rRNA Methylase in Myanmar

Antibiotic Resistance of Enterobacteriaceae Isolated from Fresh Fruits and Vegetables and Characterization of their AmpC β-Lactamases.

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