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      Blockchain of Signature Material Combining Cryptographic Hash Function and DNA Steganography

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          Abstract

          An ideal signature material and method, which can be used to prove the authenticity of a physical item and against forgery, should be immune to the fast developments in digital and engineering technologies. Herein, the design of signature material combining cryptographic hash function and DNA steganography is proposed. The encrypting materials are used to construct a series of time-stamped records (blockchain) associated with published hash values, while each DNA-encrypted block is associated with a set of DNA keys. The decrypted DNA information, as digital keys, can be validated through a hash function to compare with the published hash values. The blocks can also be cross-referenced among different related signatures. While both digital cryptography and DNA steganography can have large key size, automated brutal force search is far more labor intensive and expensive for DNA steganography with wet lab experiments, as compared to its digital counterpart. Moreover, the time-stamped blockchain structure permits the incorporation of new cryptographic functions and DNA steganographies over time, thus can evolve over time without losing the continuous history line.

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          Additive manufacturing. Continuous liquid interface production of 3D objects.

          Additive manufacturing processes such as 3D printing use time-consuming, stepwise layer-by-layer approaches to object fabrication. We demonstrate the continuous generation of monolithic polymeric parts up to tens of centimeters in size with feature resolution below 100 micrometers. Continuous liquid interface production is achieved with an oxygen-permeable window below the ultraviolet image projection plane, which creates a "dead zone" (persistent liquid interface) where photopolymerization is inhibited between the window and the polymerizing part. We delineate critical control parameters and show that complex solid parts can be drawn out of the resin at rates of hundreds of millimeters per hour. These print speeds allow parts to be produced in minutes instead of hours.
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            Enzyme-free nucleic acid logic circuits.

            Biological organisms perform complex information processing and control tasks using sophisticated biochemical circuits, yet the engineering of such circuits remains ineffective compared with that of electronic circuits. To systematically create complex yet reliable circuits, electrical engineers use digital logic, wherein gates and subcircuits are composed modularly and signal restoration prevents signal degradation. We report the design and experimental implementation of DNA-based digital logic circuits. We demonstrate AND, OR, and NOT gates, signal restoration, amplification, feedback, and cascading. Gate design and circuit construction is modular. The gates use single-stranded nucleic acids as inputs and outputs, and the mechanism relies exclusively on sequence recognition and strand displacement. Biological nucleic acids such as microRNAs can serve as inputs, suggesting applications in biotechnology and bioengineering.
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              Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.

              The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build, test, learn, and repeat cycle underpinning innovations in synthetic biology. In this perspective, we briefly review the techniques and technologies that enable the synthesis of DNA oligonucleotides and their assembly into larger DNA constructs with a focus on recent advancements that have sought to reduce synthesis cost and increase sequence fidelity. The development of lower-cost methods to produce high-quality synthetic DNA will allow for the exploration of larger biological hypotheses by lowering the cost of use and help to close the DNA read-write cost gap.
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                Author and article information

                Journal
                17 September 2019
                Article
                1909.07914
                f8213e34-3963-4e33-8d14-619846377202

                http://creativecommons.org/licenses/by/4.0/

                History
                Custom metadata
                q-bio.BM cs.CR

                Security & Cryptology,Molecular biology
                Security & Cryptology, Molecular biology

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