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      A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging

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      Author(s): , * , ,
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      Publication date (Electronic):
      Journal: Molecules
      Publisher: MDPI
      Keywords: hydrogen peroxide, reactive oxygen species, fluorescence, imaging

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          Abstract

          Hydrogen peroxide (H 2O 2) plays an important role in the human body and monitoring its level is meaningful due to the relationship between its level and diseases. A fluorescent sensor ( CMB) based on coumarin was designed and its ability for detecting hydrogen peroxide by fluorescence signals was also studied. The CMB showed an approximate 25-fold fluorescence enhancement after adding H 2O 2 due to the interaction between the CMB and H 2O 2 and had the potential for detecting physiological H 2O 2. It also showed good biocompatibility and permeability, allowing it to penetrate cell membranes and zebrafish tissues, thus it can perform fluorescence imaging of H 2O 2 in living cells and zebrafish. This probe is a promising tool for monitoring the level of H 2O 2 in related physiological and pathological research.

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          ROS function in redox signaling and oxidative stress.

          Michael Schieber, Navdeep S. Chandel (2014)
          Oxidative stress refers to elevated intracellular levels of reactive oxygen species (ROS) that cause damage to lipids, proteins and DNA. Oxidative stress has been linked to a myriad of pathologies. However, elevated ROS also act as signaling molecules in the maintenance of physiological functions--a process termed redox biology. In this review we discuss the two faces of ROS--redox biology and oxidative stress--and their contribution to both physiological and pathological conditions. Redox biology involves a small increase in ROS levels that activates signaling pathways to initiate biological processes, while oxidative stress denotes high levels of ROS that result in damage to DNA, protein or lipids. Thus, the response to ROS displays hormesis, given that the opposite effect is observed at low levels compared with that seen at high levels. Here, we argue that redox biology, rather than oxidative stress, underlies physiological and pathological conditions. Copyright © 2014 Elsevier Ltd. All rights reserved.
          Article 0 comments Cited 1219 times – based on 0 reviews     Review now
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            ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis.

            Benoit D'Autréaux, Michel B. Toledano (2007)
            Reactive oxygen species (ROS) have been shown to be toxic but also function as signalling molecules. This biological paradox underlies mechanisms that are important for the integrity and fitness of living organisms and their ageing. The pathways that regulate ROS homeostasis are crucial for mitigating the toxicity of ROS and provide strong evidence about specificity in ROS signalling. By taking advantage of the chemistry of ROS, highly specific mechanisms have evolved that form the basis of oxidant scavenging and ROS signalling systems.
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              Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling.

              Paul D Ray, Bo-Wen Huang, Yoshiaki Tsuji (2012)
              Reactive oxygen species (ROS) are generated during mitochondrial oxidative metabolism as well as in cellular response to xenobiotics, cytokines, and bacterial invasion. Oxidative stress refers to the imbalance due to excess ROS or oxidants over the capability of the cell to mount an effective antioxidant response. Oxidative stress results in macromolecular damage and is implicated in various disease states such as atherosclerosis, diabetes, cancer, neurodegeneration, and aging. Paradoxically, accumulating evidence indicates that ROS also serve as critical signaling molecules in cell proliferation and survival. While there is a large body of research demonstrating the general effect of oxidative stress on signaling pathways, less is known about the initial and direct regulation of signaling molecules by ROS, or what we term the "oxidative interface." Cellular ROS sensing and metabolism are tightly regulated by a variety of proteins involved in the redox (reduction/oxidation) mechanism. This review focuses on the molecular mechanisms through which ROS directly interact with critical signaling molecules to initiate signaling in a broad variety of cellular processes, such as proliferation and survival (MAP kinases, PI3 kinase, PTEN, and protein tyrosine phosphatases), ROS homeostasis and antioxidant gene regulation (thioredoxin, peroxiredoxin, Ref-1, and Nrf-2), mitochondrial oxidative stress, apoptosis, and aging (p66Shc), iron homeostasis through iron-sulfur cluster proteins (IRE-IRP), and ATM-regulated DNA damage response. Copyright © 2012 Elsevier Inc. All rights reserved.
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                Author and article information

                Contributors
                Mei Pan: Role: Academic Editor
                Journal
                Journal ID (nlm-ta): Molecules
                Journal ID (iso-abbrev): Molecules
                Journal ID (publisher-id): molecules
                Title: Molecules
                Publisher: MDPI
                ISSN (Electronic): 1420-3049
                Publication date (Electronic): 02 June 2021
                Publication date Collection: June 2021
                Volume: 26
                Issue: 11
                Electronic Location Identifier: 3352
                Affiliations
                State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China; z943877035@ 123456mail.dlut.edu.cn (Y.Z.); sxcm98@ 123456gmail.com (C.M.); cyduan@ 123456dlut.edu.cn (C.D.)
                Author notes
                [* ]Correspondence: jiaoyang@ 123456dlut.edu.cn
                Author information
                https://orcid.org/0000-0001-8927-9367
                Article
                Publisher ID: molecules-26-03352
                DOI: 10.3390/molecules26113352
                PMC ID: 8199646
                PubMed ID: 34199465
                SO-VID: fb5bd2ed-02b0-4465-b423-62bb966e5426
                Copyright © © 2021 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 01 May 2021
                Date accepted : 28 May 2021
                Categories
                Subject: Article

                Keywords: hydrogen peroxide,reactive oxygen species,fluorescence,imaging
                Data availability:
                Keywords: hydrogen peroxide, reactive oxygen species, fluorescence, imaging

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