Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration

Abstract

Bone defects are a common challenge in the clinical setting. Bone tissue engineering (BTE) is an effective treatment for the clinical problem of large bone defects. In this study, we fabricated silk fibroin (SF)/hydroxyapatite (HAp) scaffolds inlaid with naringin poly lactic-co-glycolic acid (PLGA) microspheres, investigating the feasibility of their application in BTE. Naringin PLGA microspheres were manufactured and adhered to the SF/HAp scaffold. Bone mesenchymal stem cells (BMSCs) were inoculated onto the SF/HAp scaffold containing naringin PLGA microsphere to examine the biocompatibility of the SF/HAp scaffolds. A rabbit femoral distal bone defect model was used to evaluate the in vivo function of the SF/HAp scaffolds containing naringin-loaded PLGA microspheres. The current study demonstrated that SF/HAp scaffolds containing naringin-loaded PLGA microspheres show promise as osteo-modulatory biomaterials for bone regeneration.

Graphical abstract

Overall schematic illustration of naringin microsphere silk fibroin/hydroxyapatite scaffold enhances BMSCs-based bone regeneration.

Highlights

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  A novel porous composite scaffold was fabricated with naringin, SF and HAp.

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  MSN/SF/HAp scaffold shows promise as an osteo-modulatory biomaterial for bone regeneration.

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  MSN/SF/HAp scaffold exhibited osteogenic differentiation potential and is a promising candidate for tissue engineering.