8
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice.

      Proceedings of the National Academy of Sciences of the United States of America
      Animals, B-Lymphocytes, immunology, Lymphocyte Culture Test, Mixed, Lymphoid Tissue, cytology, Macrophages, Major Histocompatibility Complex, Mice, T-Lymphocytes

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Dendrite cells (DCs) are a new cell type initially identified in mouse lymphoid organs. Recently, DCs have been purified from mouse spleen. This paper demonstrates a functional role of DCs: they are potent stimulators of the primary mixed leukocytes reaction (MLR). As few as 300-1000 DCs doubled the proliferative activity of 5 X 10(6) allogeneic responder spleen cells, while 0.3-1.0 X 10(5) DCs induced a maximal stimulation of 30- to 80-fold. Between these extremes, the log of the MLR response increased linearly with the log of DC numbers. This dose-response assay was then used to compare the potency of purified DCs with that of other heterogeneous lymphoid populations, many of which gave dose-response curves with similar slopes. The potency of purified DCs as MLR stimulators was 100-300 times greater than that of unfractionated spleen cells. When spleen cells were fractionated by simple physical techniques, MLR-stimulating capacity in the subpopulations correlated closely with DC numbers. Removal of splenic B or T lymphocytes, by anti-immunoglobulin or anti-brain serum plus complement, did not reduce MLR-stimulating capacity. Finally, several populations, enriched in mononuclear phagocytes but lacking in DCs, stimulated weakly if at all. We conclude that DCs are a potent stimulating cell and are at least 100 times more effective than other major cell subclasses--i.e., B and T lymphocytes and macrophages.

          Related collections

          Author and article information

          Journal
          154105
          336278
          10.1073/pnas.75.10.5132

          Chemistry
          Animals,B-Lymphocytes,immunology,Lymphocyte Culture Test, Mixed,Lymphoid Tissue,cytology,Macrophages,Major Histocompatibility Complex,Mice,T-Lymphocytes

          Comments

          Comment on this article