Journal ID (nlm-journal-id): 101186374
Journal ID (pubmed-jr-id): 31761
Journal ID (nlm-ta): Nat Struct Mol Biol
Title:
Nature structural & molecular biology
ISSN
(Print):
1545-9993
ISSN
(Electronic):
1545-9985
Publication date Nihms-submitted: 12
November
2010
Publication date
(Electronic):
16
January
2011
Publication date
(Print):
February
2011
Publication date PMC-release: 1
August
2011
Volume: 18
Issue: 2
Pages: 198-204
Affiliations
[1
]Division of Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066
CX Amsterdam, The Netherlands
[2
]Pfizer Global Research and Development, Chesterfield, MO 63017, USA
[3
]Division of Cardiovascular Medicine, University of Kentucky, Lexington, KY 40536,
USA
[4
]Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066
CX Amsterdam, The Netherlands
[5
]Laboratory of Biosignaling & Therapeutics, Department of Molecular Cell Biology, University
of Leuven, B-3000 Leuven, Belgium
[6
]Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University
of Oxford, Oxford, UK
Author notes
[7]
These authors contributed equally to this work
J.H. purified, crystallized and collected diffraction data for native crystal forms;
established co-crystals, collected diffraction data and participated in structure
determination of the HA155 complex; and cloned, expressed and assayed activity of
catalytic and binding site mutants; S.K. directly supervised Pfizer scientists, collected
and processed diffraction data, determined the first structure, built and refined
crystal structures; E.C. established cell lines, protein expression and purification,
crystallization protocols for the first native crystal form, and collected diffraction
data; J.E.D. identified and produced the second, high resolution, native crystal form
and collected diffraction data; T.W. and Z.F. generated all reagents for ATX platelet
binding experiments and performed the relevant assays; L.v.M participated in protein
expression and with H.M.H.G.A. and A.H help to establish ATX assays; H.M.H.G.A made
the HA155 inhibitor; L.v.Z contributed the PAI-1 experiment; S.J. generated the
rATX mutant used for crystallization, M.A. performed the EF-hand-like mutagenesis experiments
and assays; L.E.P. expressed protein; T.E.B. coordinated the Pfizer scientists; K.H
assisted with crystal growth, manipulation and data collection; M.K. helped establish
the stable cell line producing
rATX; CvdK, S.S.S and A.J.M. designed and supervised platelet binding experiments;
H.O. supervised inhibitor design at the NKI site; M.B. supervised EF-hand experiments
and participated in project coordination; W.H.M. and A.P. initiated and coordinated
the project and supervised experiments at the NKI; A.P. also determined and refined
crystal structures, prepared all display items, and wrote the paper with input from
W.H.M., S.K., M.B. and A.J.M.
Article
Manuscript ID: nihpa251225
DOI: 10.1038/nsmb.1980
PMC ID: 3064516
PubMed ID: 21240271
SO-VID: 18a00078-d5ea-4b42-9425-f5c8bc04f3cc
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Funded by: National Heart, Lung, and Blood Institute : NHLBI
Funded by: National Institute of General Medical Sciences : NIGMS
Funded by: National Center for Research Resources : NCRR
Award ID: R01 HL078663-06
||HL
Funded by: National Heart, Lung, and Blood Institute : NHLBI
Funded by: National Institute of General Medical Sciences : NIGMS
Funded by: National Center for Research Resources : NCRR
Award ID: R01 GM050388-18
||GM
Funded by: National Heart, Lung, and Blood Institute : NHLBI
Funded by: National Institute of General Medical Sciences : NIGMS
Funded by: National Center for Research Resources : NCRR
Award ID: P20 RR021954-03
||RR
