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      Recent trends in click chemistry as a promising technology for virus-related research

      review-article
      , , , *
      Virus Research
      Elsevier B.V.
      Click chemistry, Virus, Labeling, Biosensor, Delivery, Diagnosis

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          Highlights

          • Click chemistry describes reactions used to generate substances by joining small units together with heteroatom linkages.

          • Biosensors combined with analytical devices is convenient strategy for viral detection.

          • We reviewed the recent applications of click reactions in virus-related research.

          • This review provides an overview of the general principles and applications of click chemistry in virus-related research.

          Abstract

          Click chemistry involves reactions that were originally introduced and used in organic chemistry to generate substances by joining small units together with heteroatom linkages (C-X-C). Over the last few decades, click chemistry has been widely used in virus-related research. Using click chemistry, the virus particle as well as viral protein and nucleic acids can be labeled. Subsequently, the labeled virions or molecules can be tracked in real time. Here, we reviewed the recent applications of click reactions in virus-related research, including viral tracking, the design of antiviral agents, the diagnosis of viral infection, and virus-based delivery systems. This review provides an overview of the general principles and applications of click chemistry in virus-related research.

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          Most cited references75

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          Thiol-ene click chemistry.

          Following Sharpless' visionary characterization of several idealized reactions as click reactions, the materials science and synthetic chemistry communities have pursued numerous routes toward the identification and implementation of these click reactions. Herein, we review the radical-mediated thiol-ene reaction as one such click reaction. This reaction has all the desirable features of a click reaction, being highly efficient, simple to execute with no side products and proceeding rapidly to high yield. Further, the thiol-ene reaction is most frequently photoinitiated, particularly for photopolymerizations resulting in highly uniform polymer networks, promoting unique capabilities related to spatial and temporal control of the click reaction. The reaction mechanism and its implementation in various synthetic methodologies, biofunctionalization, surface and polymer modification, and polymerization are all reviewed.
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            A chemical method for fast and sensitive detection of DNA synthesis in vivo.

            We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the EdU label is highly sensitive and can be accomplished in minutes. The small size of the fluorescent azides used for detection results in a high degree of specimen penetration, allowing the staining of whole-mount preparations of large tissue and organ explants. In contrast to BrdU, the method does not require sample fixation or DNA denaturation and permits good structural preservation. We demonstrate the use of the method in cultured cells and in the intestine and brain of whole animals.
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              • Record: found
              • Abstract: not found
              • Article: not found

              Click chemistry for drug development and diverse chemical-biology applications.

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                Author and article information

                Contributors
                Journal
                Virus Res
                Virus Res
                Virus Research
                Elsevier B.V.
                0168-1702
                1872-7492
                3 August 2018
                2 September 2018
                3 August 2018
                : 256
                : 21-28
                Affiliations
                [0005]Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun, 130062, China
                Author notes
                [* ]Corresponding author. renlz@ 123456jlu.edu.cn
                Article
                S0168-1702(18)30352-6
                10.1016/j.virusres.2018.08.003
                7173221
                30081058
                22a65509-fe9b-4d41-9cff-1dbfbfa00d38
                © 2018 Elsevier B.V. All rights reserved.

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                Categories
                Article

                Microbiology & Virology
                click chemistry,virus,labeling,biosensor,delivery,diagnosis
                Microbiology & Virology
                click chemistry, virus, labeling, biosensor, delivery, diagnosis

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