12
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Unique and overlapping functions of the Exo1, Mre11 and Pso2 nucleases in DNA repair.

      DNA Repair

      DNA Damage, DNA Repair, Endodeoxyribonucleases, genetics, metabolism, Exodeoxyribonucleases, Gamma Rays, Methyl Methanesulfonate, toxicity, Mutagens, Radiation Tolerance, Saccharomyces cerevisiae, drug effects, enzymology, radiation effects, Saccharomyces cerevisiae Proteins

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The Mre11 and Pso2 nucleases function in homologous recombination and interstrand cross-link (ICL) repair pathways, respectively, while the Exo1 nuclease is involved in homologous recombination and mismatch repair. Characterization of the sensitivity of single, double and triple mutants for these nucleases in Saccharomyces cerevisiae to various DNA damaging agents reveals complex interactions that depend on the type of DNA damage. The pso2 mutant is uniquely sensitive to agents that generate ICLs and mre11-H125N shows the highest sensitivity of the single mutants for ionizing radiation and methyl methane sulfonate. However, elimination of all three nucleases confers higher sensitivity to IR than any of the single or double mutant combinations indicating a high degree of redundancy and versatility in the response to DNA damage. In response to ICL agents, double-strand breaks are still formed in the triple nuclease mutant indicating that none of these nucleases are responsible for unhooking cross-links.

          Related collections

          Author and article information

          Journal
          18295552
          2349096
          10.1016/j.dnarep.2007.12.014

          Comments

          Comment on this article