Wisteria floribunda agglutinin (WFA) is a lectin that labels selectively N-acetylgalactosamines beta 1 (GalNAc beta 1-3 Gal) residues of glycoproteins within the extracellular matrix of the neurons, and has been identified as a specific marker for functionally different cortical areas of the rodent brain. Here we report that WFA-binding sites can be used for the characterization of cortical areas and their subdivisions of the immersion-fixed human brain. WFA-binding showed an area-specific distribution pattern within areas 1, and 3a-3b of the somatosensory cortex as well as in the primary motor areas 4a-4p. The WFA-binding labeled stripes of 150-175 microm width at intervals of 800-1000 microm within the motor cortex but not in the somatosensory cortex. At the cellular level, differences in staining intensities among certain cell types were evident among WFA-positive glial cells. WFA binding seems to be a useful marker to reveal areal borders and function related intraareal specializations in combination with immunocytochemical techniques.