Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition.

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Abstract

The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.

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Author and article information

Journal

Journal ID (iso-abbrev): Virology

Title: Virology

Publisher: Elsevier BV

ISSN (Electronic): 1096-0341

ISSN (Print): 0042-6822

Publication date (Electronic): Dec 2016

Volume: 499

Affiliations

[1 ] Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.

[2 ] Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China; Shandong Institute of Veterinary Drug Quality Inspection, Shandong Key Laboratory for Quality Safety Monitoring and Risk Assessment of Animal Products, Huaicun Street No. 68, Jinan 250722, Shandong Province, China.

[3 ] Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802, USA.

[4 ] Department of Biology and Microbiology, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.

[5 ] College of marine science and engineering, Qingdao Agricultural University, Changcheng Road No. 700, Qingdao 266109, China.

[6 ] Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: wujiaqiang2000@sina.com.

[7 ] Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: duyijun0916@163.com.

[8 ] Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: wangjb@saas.ac.cn.

Article

Publisher Item ID: S0042-6822(16)30176-3

DOI: 10.1016/j.virol.2016.07.008

PubMed ID: 27718457

SO-VID: 2da1c850-cabe-4016-bd1b-154e9e4954eb

History

Keywords: Nuclear export signal (NES),PRRSV,Type I IFN,NSP1α

Data availability:

Keywords: Nuclear export signal (NES), PRRSV, Type I IFN, NSP1α

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