The Morris water maze, a spatial learning task first introduced by Dr. Richard Morris (Morris, 1981, 1984), is one of the most extensively used behavioral paradigms used to examine hippocampus-dependent spatial learning and memory in rodents, such as rats and mice (Tanda et al., 2009). In water maze tests, animals are forced to swim in a circular pool of milky water. In order to escape from the water, the animals are required to locate a submerged hidden platform with the aid of spatial cues surrounding the water pool. Once practiced, the animals begin to learn the relationship between the platform location and various spatial cues, and eventually form a memory of the location of the hidden platform (Morris, 1984). To determine whether animals acquire a spatial learning about the location of the hidden platform and are able to search for the platform solely by relying on spatial memory, probe trials are performed where the escape platform is removed from the pool and the animals are permitted to swim and search the pool for 1 min. In the probe trial, the occupational time spent in the specific quadrant where the platform was originally placed and the number of crossings through the former hidden platform location are used as general measures to quantify the level of spatial memory. Since its development, the water maze has become the core assay of behavioral test batteries used to analyze hippocampus-dependent brain functions, and has subsequently been exploited to explore the functions of other areas of the brain (Tsien et al., 1996; D'Hooge and De Deyn, 2001). Moreover, a variety of protocols, including reversal training and delayed matching-to-place paradigm, have been established for investigating the role of specific genes or proteins in normal and abnormal brain functions (Tsien et al., 1996; Gallagher and Rapp, 1997; Lipp and Wolfer, 1998; Vorhees and Williams, 2006). In contrast to this progress, however, few studies have questioned the effectiveness of the existing analytic measures, such as percentage quadrant time, percentage zone, and platform crossings, that are used in water maze studies. Furthermore, relatively few attempts have been made to improve the sensitivity of the water maze in order to detect possible subtle phenotypic changes that might occur between groups during learning (Maei et al., 2009). In a research article published in Frontiers in Neuroscience, Maei et al. (2009) describe a newly developed analytical method for detecting group differences more sensitively and accurately in the water maze. Herein, the authors introduced the concept of entropy (H) – a measure of the disorder of a system. The rationale underlying this idea is that over the course of water maze training, an animal's search strategy might be shifted from disorganized to more focal searching (Vorhees and Williams, 2006), which can be considered as a reduction of disorder in the system. By using entropy (H) as an analytical measure for the water maze, Maei et al. (2009) argue that we can more fully exploit an animal's positional data produced by tracking software, thereby providing greater sensitivity for detecting phenotypic differences. To evaluate how H works and to compare the usefulness of H with existing measures, the authors rendered a series of Monte Carlo simulations and randomly selected individual trials from a dataset of more than 1600 probe tests. This approach allowed them to simulate water maze experiments with varying sample and effect sizes. Next, by summing two types of variance – error variance (the variance of an animal's position with respect to the target; Herror) and path variance (the variance of an animal's position with respect to its path; Hpath) – and using the summed value as a measure of the entropy of spatial navigation, H could thoroughly utilize the positional information from each tracking record. Lastly, they experimentally verified H using three paradigms of hippocampal dysfunctions. The authors report that H outperforms existing measures in terms of its sensitivity in detecting group differences over a range of sample or effect sizes. As discussed by the authors, the excellence of H can be recapitulated by two points. One concerns its full usage of precise positional information of an animal throughout the probe test. Represented by Herror and Hpath, the H measure assesses both the degree to which searching is focused on the former platform location and how focused the search is. Using this approach, H can hold a greater amount of detailed trajectory information than any other measure, which confers exceptional sensitivity upon H, particularly for comparisons between subjects. In addition, by adjusting the relative weight between Herror and Hpath, H can be adjusted to outperform other measures under various experimental conditions. The second point is that the H measure turns out to be distributed normally, which is in stark contrast to other measures (Maei et al., 2009). This characteristic gives a further advantage to the H measure because normality is a prerequisite for parametric tests, such as the t-test and ANOVA, to yield correct statistical results without type-1 errors. Moreover, parametric tests are the most widely used statistical methods for analyzing behavioral test batteries. To summarize, the analytical approach described by Maei et al. (2009) provides a more effective and accurate new measure in terms of its superb sensitivity and statistical validity in water maze studies. Furthermore, the H measure will contribute greatly to the advancement of behavioral assays that, in conjunction with molecular genetic approaches, are designed to elucidate the molecular mechanisms underlying learning and memory.