Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila

Immunostimulant preparations Macrogard, Candida utilis, Saccharomyces cerevisiae, Evetsel, Chitosan, or FinnStim were mixed into semipurified diets and fed to groups of rainbow trout for 1 week. Fish were bled by non-lethal caudal puncture and blood samples assayed for changes in non-specific cellular immunity and humoral protein levels. In the immunostimulated fish, hematocrit levels and lymphocyte counts remained relatively stable; however, elevations were observed in oxidative radical release, myeloperoxidase activity, phagocytic indexes, and potential killing activities of phagocytic cells including neutrophils. Total plasma protein and total immunoglobulin levels were elevated by the dietary immunostimulants. A challenge with the virulent pathogen that causes furunculosis, Aeromonas salmonicida, showed that the immunostimulated groups of fish were more resistant to the disease, confirming the potential use of these substances in fish culture for the prevention of disease.