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      High-Quality Genome Sequence of Bacillus anthracis Strain 14RA5914 Isolated during an Outbreak in Germany in 2014

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          ABSTRACT

          Bacillus anthracis is a zoonotic agent causing anthrax, a notifiable disease in animals. The last anthrax outbreak among cattle in Germany occurred in April 2014 in Saxony-Anhalt. Here we report a high-quality genome sequence of the Bacillus anthracis strain 14RA5914 Dobichau isolated from the spleen of a dead cow.

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          A multiplex real-time PCR for identifying and differentiating B. anthracis virulent types.

          Tim Brooks, Mary Jane Burton, Ingmar Janse … (2011)
          Bacillus anthracis is closely related to the endospore forming bacteria Bacillus cereus and Bacillus thuringiensis. For accurate detection of the life threatening pathogen B. anthracis, it is essential to distinguish between these three species. Here we present a novel multiplex real-time PCR for simultaneous specific identification of B. anthracis and discrimination of different B. anthracis virulence types. Specific B. anthracis markers were selected by whole genome comparison and different sets of primers and probes with optimal characteristic for multiplex detection of the B. anthracis chromosome, the B. anthracis pXO1 and pXO2 plasmids and an internal control (IC) were designed. The primer sets were evaluated using a panel of B. anthracis strains and exclusivity was tested using genetically closely related B. cereus strains. The robustness of final primer design was evaluated by laboratories in three different countries using five different real-time PCR thermocyclers. Testing of a panel of more than 20 anthrax strains originating from different locations around the globe, including the recent Swedish anthrax outbreak strain, showed that all strains were detected correctly. Copyright © 2010 Elsevier B.V. All rights reserved.
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            Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR.

            Timothy Straub, Cheryl Baird, Rachel Bartholomew … (2013)
            We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR.
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              Draft genome sequence of Bacillus anthracis BF-1, isolated from Bavarian cattle.

              Jörn Kalinowski, Matthias Hanczaruk, Gregor Grass … (2012)
              Bacillus anthracis BF-1 was isolated from a cow in Bavaria (Germany) that had succumbed to anthrax. Here, we report the draft genome sequence of this strain, which belongs to the European B2 subclade of B. anthracis. The closest phylogenetic neighbor of strain BF-1 is a strain isolated from cattle in France.
              Article 0 comments Cited 8 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Genome Announc
                Journal ID (iso-abbrev): Genome Announc
                Journal ID (hwp): ga
                Journal ID (pmc): ga
                Journal ID (publisher-id): GA
                Title: Genome Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2169-8287
                Publication date (Electronic): 5 October 2017
                Publication date Collection: October 2017
                Volume: 5
                Issue: 40
                Electronic Location Identifier: e01002-17
                Affiliations
                [a ]Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany
                [b ]Department of Veterinary Medicine, State Institute for Consumer Protection of Saxony-Anhalt, Stendal, Germany
                Author notes
                Address correspondence to Mandy C. Elschner, mandy.elschner@ 123456fli.de .

                M.C.E. and A.B. contributed equally to this work.

                Article
                Publisher ID: genomeA01002-17
                DOI: 10.1128/genomeA.01002-17
                PMC ID: 5629050
                PubMed ID: 28982993
                SO-VID: 37e2cae6-7095-4652-a3d9-3154b7a7edda
                Copyright © Copyright © 2017 Elschner et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 25 August 2017
                Date accepted : 31 August 2017
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 8, Pages: 2, Words: 1084
                Categories
                Subject: Prokaryotes
                Custom metadata
                cover-date October 2017

                ScienceOpen disciplines: Genetics
                Data availability:
                ScienceOpen disciplines: Genetics

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