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Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution.

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Biochimica et biophysica acta

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      Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the following, recent examples of the how different versions of this analytical approach, namely reverse phase liquid chromatography-multiple reaction monitoring, normal phase liquid chromatography/tandem mass spectrometry and normal phase liquid chromatography-precursor ion scan detection have respectively served to address novel aspects of dolichol or polyprenol biology in Eukarya, Archaea and Bacteria.

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      [1 ] Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
      Biochim. Biophys. Acta
      Biochimica et biophysica acta
      Nov 2011
      : 1811
      : 11
      21570481 S1388-1981(11)00061-8 10.1016/j.bbalip.2011.04.009 3419941 NIHMS396531
      Copyright © 2011 Elsevier B.V. All rights reserved.


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