Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

Abstract

Simple Summary

Intensive selection in broiler chicken flocks has led do several leg disorders. The injection of nanoparticles, with high specificity to the bone, into the egg is a potential method to improve bone quality. The objective of our study was to evaluate the potential effect of calcium carbonate nanoparticles injected to the egg on chicken embryo development and bone quality of broiler chickens after 42 day of life. The calcium carbonate nanoparticles were not toxic to embryo and even improved the bone quality of embryos and later broilers without negative impact on production results. Thus, the application of calcium carbonate nanoparticles to the egg may be the potential solution for improving the bone mineralization of broiler chickens.

Abstract

The use of intensive selection procedure in modern broiler chicken lines has led to the development of several skeletal disorders in broiler chickens. Therefore, current research is focused on methods to improve the bone quality in birds. In ovo technology, using nanoparticles with a high specificity to bones, is a potential approach. The present study aimed to evaluate the effect of in ovo inoculation (IOI) of calcium carbonate nanoparticles (CCN) on chicken embryo development, health status, bone characteristics, and on broiler production results and bone quality. After assessing in vitro cell viability, the IOI procedure was performed with an injection of 500 μg/mL CCN. The control group was not inoculated with CCN. Hatchability, weight, and selected bone and serum parameters were measured in embryos. Part of hatchlings were reared under standard conditions until 42 days, and production results, meat quality, and bone quality of broilers were determined. CCN did not show cytotoxicity to cells and chicken embryo and positively influenced bone parameters of the embryos and of broilers later (calcification) without negatively affecting the production results. Thus, the IOI of CCN could modify the molecular responses at the stage of embryogenesis, resulting in better mineralization, and could provide a sustained effect, thereby improving bone quality in adult birds.