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      Simultaneous angular multiplexing optical projection tomography at shifted focal planes.

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          Abstract

          We describe an angular multiplexing technique for optical projection tomography that improves resolution, signal-to-noise ratio, and imaging speed by ameliorating the trade-off between spatial resolution and depth of field and improving the light collection efficiency. Here we demonstrate that imaging at two orthogonal angular projections simultaneously, focused on shifted planes in the sample, improves the average spatial resolution by ~20% and the light collection efficiency by a factor of ~4, thereby enabling increased acquisition speed and reduced light dose.

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          Author and article information

          Journal
          Opt Lett
          Optics letters
          1539-4794
          0146-9592
          Mar 15 2013
          : 38
          : 6
          Affiliations
          [1 ] Photonics Group, Department of Physics, Imperial College London, London, UK. l.chen10@imperial.ac.uk
          Article
          250646
          10.1364/OL.38.000851
          23503237
          4061dc1e-41a7-4e46-bd85-9976ce3e81a2
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