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      High-speed polarization sensitive optical coherence tomography scan engine based on Fourier domain mode locked laser

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          Abstract

          We report on a new swept source polarization sensitive optical coherence tomography scan engine that is based on polarization maintaining (PM) fiber technology. The light source is a Fourier domain mode locked laser with a PM cavity that operates in the 1300 nm wavelength regime. It is equipped with a PM buffer stage that doubles the fundamental sweep frequency of 54.5 kHz. The fiberization allows coupling of the scan engine to different delivery probes. In a first demonstration, we use the system for imaging human skin at an A-scan rate of 109 kHz. The system illuminates the sample with circularly polarized light and measures reflectivity, retardation, optic axis orientation, and Stokes vectors simultaneously. Furthermore, depolarization can be quantified by calculating the degree of polarization uniformity (DOPU). The high scanning speed of the system enables dense sampling in both, the x- and y-direction, which provides the opportunity to use 3D evaluation windows for DOPU calculation. This improves the spatial resolution of DOPU images considerably.

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          Most cited references52

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          Optical coherence tomography.

          A technique called optical coherence tomography (OCT) has been developed for noninvasive cross-sectional imaging in biological systems. OCT uses low-coherence interferometry to produce a two-dimensional image of optical scattering from internal tissue microstructures in a way that is analogous to ultrasonic pulse-echo imaging. OCT has longitudinal and lateral spatial resolutions of a few micrometers and can detect reflected signals as small as approximately 10(-10) of the incident optical power. Tomographic imaging is demonstrated in vitro in the peripapillary area of the retina and in the coronary artery, two clinically relevant examples that are representative of transparent and turbid media, respectively.
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            Optical coherence tomography - principles and applications

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              Characterization of human atherosclerosis by optical coherence tomography.

              High-resolution visualization of atherosclerotic plaque morphology may be essential for identifying coronary plaques that cause acute coronary events. Optical coherence tomography (OCT) is an intravascular imaging modality capable of providing cross-sectional images of tissue with a resolution of 10 micro m. To date, OCT imaging has not been investigated in sufficient detail to assess its accuracy for characterizing atherosclerotic plaques. The aim of this study was to establish objective OCT image criteria for atherosclerotic plaque characterization in vitro. OCT images of 357 (diseased) atherosclerotic arterial segments obtained at autopsy were correlated with histology. OCT image criteria for 3 types of plaque were formulated by analysis of a subset (n=50) of arterial segments. OCT images of fibrous plaques were characterized by homogeneous, signal-rich regions; fibrocalcific plaques by well-delineated, signal-poor regions with sharp borders; and lipid-rich plaques by signal-poor regions with diffuse borders. Independent validation of these criteria by 2 OCT readers for the remaining segments (n=307) demonstrated a sensitivity and specificity ranging from 71% to 79% and 97% to 98% for fibrous plaques, 95% to 96% and 97% for fibrocalcific plaques, and 90% to 94% and 90% to 92% for lipid-rich plaques, respectively (overall agreement, kappa=0.83 to 0.84). The interobserver and intraobserver reliabilities of OCT assessment were high (kappa values of 0.88 and 0.91, respectively). Objective OCT criteria are highly sensitive and specific for characterizing different types of atherosclerotic plaques. These results represent an important step in validating this new intravascular imaging modality and will provide a basis for the interpretation of intracoronary OCT images obtained from patients.
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                Author and article information

                Journal
                Biomed Opt Express
                Biomed Opt Express
                BOE
                Biomedical Optics Express
                Optical Society of America
                2156-7085
                25 October 2012
                01 November 2012
                25 October 2012
                : 3
                : 11
                : 2987-3000
                Affiliations
                [1 ]Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, A-1090 Vienna, Austria
                [2 ]Lehrstuhl für BioMolekulare Optik, Fakultät für Physik, Ludwig-Maximilians-Universität München, D-80538 München, Germany
                Author notes
                Article
                176564
                10.1364/BOE.3.002987
                3493228
                23162734
                6bb4efea-ddea-40c7-8caf-99b9bd5d0498
                ©2012 Optical Society of America

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

                History
                : 20 September 2012
                : 23 October 2012
                : 23 October 2012
                Funding
                Funded by: European Union (project FUN OCT, FP7 HEALTH
                Award ID: 201880
                Categories
                Optical Coherence Tomography
                Custom metadata
                True
                0

                Vision sciences
                (170.4580) optical diagnostics for medicine,(230.5440) polarization-selective devices,(170.4500) optical coherence tomography

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