In this study, the experimental denture base on the hard palate of the molar region of osteoporotic rats was designed to cover without any mechanical pressure or to load continuous pressure (0.5, 1.5 or 3.4 kPa). Experimental osteoporosis was induced by ovariectomy and feeding on a low calcium diet. Histopathological changes induced by non-pressure covering or continuous pressure in the tissues under the experimental denture bases were examined. Regarding the histological changes induced by continuous pressure, this was evaluated in relation to the time course of the intensity of the continuous pressure. In the tissues under the denture base in osteoporotic condition with non-pressure covering, no histopathological changes caused by non-pressure covering were observed except for a transient slight proliferative reaction in the epithelial ridge. The following histopathological changes were observed in the tissues under the denture base in the osteoporotic condition bearing continuous pressure; (i) bone resorption was induced on the periosteal surface (Ps) in the osteoporotic-3.4 kPa group, and on the pressure side of the endocortical surface (p-Ec) in the osteoporotic-1.5 and -3.4 kPa group, (ii) the appearance of osteoclasts on the p-Ec in the osteoporotic-3.4 kPa group was earlier than that on the Ps in a same group, (iii) bone resorption on the pressure side of the Ec in the osteoporotic-3.4 kPa group manifested at the earlier stage than that in the osteoporotic-1.5 kPa group, (iv) bone formation following bone resorption on the p-Ec was observed more markedly than that on the Ps and (v) histopathological changes in the tissues under the denture base in experimental osteoporosis bearing continuous pressure had an intimate relationship with the initial intensity and the time course of continuous pressure.