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Conservation In vitro of threatened plants—Progress in the past decade

Author(s): Viswambharan Sarasan , Ryan Cripps , Marcaret M. Ramsay , Caroline Atherton , Monica McMichen , Grace Prendergast , Jennifer K. Rowntree

Publication date Created: May 2006

Publication date (Print): May 2006

Journal: In Vitro Cellular & Developmental Biology - Plant

Publisher: CABI Publishing

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Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.

A Sakai, I Oiyama, Koichi Kobayashi (1990)

The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.

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Plant cryopreservation: Progress and prospects

Florent Engelmann (2004)

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Constraints to symbiotic germination of terrestrial orchid seed in a mediterranean bushland

K W Dixon, M. Brundrett, K Sivasithamparam … (2001)

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Author and article information

Journal

Title: In Vitro Cellular & Developmental Biology - Plant

Abbreviated Title: In Vitro Cell. Dev. Biol. - Plant

Publisher: CABI Publishing

ISSN (Print): 1054-5476

ISSN (Electronic): 1475-2689

Publication date Created: May 2006

Publication date (Print): May 2006

Volume: 42

Issue: 3

Pages: 206-214

Article

DOI: 10.1079/IVP2006769

SO-VID: 48370f1c-06c2-46c7-bbd1-60a4912d66fa

Copyright © © 2006

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