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      Conservation In vitro of threatened plants—Progress in the past decade

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          Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.

          The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.
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            Plant cryopreservation: Progress and prospects

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              Constraints to symbiotic germination of terrestrial orchid seed in a mediterranean bushland

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                Author and article information

                Journal
                In Vitro Cellular & Developmental Biology - Plant
                In Vitro Cell. Dev. Biol. - Plant
                CABI Publishing
                1054-5476
                1475-2689
                May 2006
                May 2006
                : 42
                : 3
                : 206-214
                Article
                10.1079/IVP2006769
                48370f1c-06c2-46c7-bbd1-60a4912d66fa
                © 2006
                History

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